Purification of Extracellular Protease Produced by Rhizoctonia solani and Its Partial Characterization  

水稻纹枯病菌胞外蛋白酶分离纯化及部分特性分析(英文)

作　　者：范文艳[1] 陈瑾[1] 文景芝[2] 马建[1] 石园园[1] 

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163319 [2]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《Plant Diseases and Pests》2010年第1期5-8,共4页植物病虫害研究（英文版）

基　　金：Supported by High Academic Youth Backbone Support Projects in Heilongjiang Province(1152G022);Scientific Research Foundation for Doctor in Heilongjiang August First Land Reclamation University~~

摘　　要：[Objective] The aim of the study was to provide the basis for researching the pathogenicity mechanism of Rhizoctonia solani.[Method] The extracellular protease was purified after ammonium sulfate precipitation through DEAE-Sephrase Fast Flow,Phenyl-Sepharose Fast Flow and Sephadex G-75 ch rom atography. [Result] The extracellular protease with molecular weight of 49.5 ku was obtained from fermentation liquid of R. solani. The optimal temperature and pH value for its activity were 6.4 and 30 ℃ respectively. Zn^2＋,Fe^3＋,Cu^2＋had inhibition on enzyme activity,while Mg^2＋,Mn^2＋had no effect on enzyme activity,and Ca^2＋ could activate enzymatic activity in low concentration.[Conclusion] R. solani could secrete extracellular protease,but the relationship between the extracellular protease and the pathogenicity of R. solani required further study.[Objective] The aim of the study was to provide the basis for researching the pathogenicity mechanism of Rhizoctonia solani.[Method] The extracellular protease was purified after ammonium sulfate precipitation through DEAE-Sephrase Fast Flow,Phenyl-Sepharose Fast Flow and Sephadex G-75 ch rom atography. [Result] The extracellular protease with molecular weight of 49.5 ku was obtained from fermentation liquid of R. solani. The optimal temperature and pH value for its activity were 6.4 and 30 ℃ respectively. Zn^2＋,Fe^3＋,Cu^2＋had inhibition on enzyme activity,while Mg^2＋,Mn^2＋had no effect on enzyme activity,and Ca^2＋ could activate enzymatic activity in low concentration.[Conclusion] R. solani could secrete extracellular protease,but the relationship between the extracellular protease and the pathogenicity of R. solani required further study.

关 键 词：Rhizoctonia solani Kuhn Extracellular protease PATHOGENICITY 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治]