Separation and Screening of Biocontrol Actinomyces against Alfalfa Root Rot  

紫花苜蓿根腐病性病害生防放线菌的分离与筛选(英文)

作　　者：王兰英[1] 徐林波[2] 何勇 乌兰巴特尔 

机构地区：[1]海南大学环境与植物保护学院,海南儋州571737 [2]中国农业科学院草原研究所,内蒙古呼和浩特010010 [3]青岛海源草坪有限公司,山东青岛026300

出　　处：《Plant Diseases and Pests》2010年第2期10-12,37,共4页植物病虫害研究（英文版）

基　　金：Supported by Central Nonprofit Research Institutions Basic Scientific Research Operating Expenses(Grassland Research Institute,Chinese Academy of Agricultural Sciences2006-01-05)~~

摘　　要：[Objective] Actinomycetes with high antagonistic effects on alfalfa root rot pathogen was isolated from 10 soil samples in Chifeng Inner Mongolia. [Method] 91 actinomyces were separated from 10 soil samples in Chifeng Inner Mongolia by gradient dilution separation method. In duel culture tests, all isolates were tested for their antagonism by using 3 strains including Fusarium solani, F. oxysoporum, F. avenaceum of alfalfa root rot pathogenic bacteria as indicator strains. [Result] 5 strains with strong antagonistic effect on tested alfalfa root rot pathogen were obtained from No.1, No.4, No.6 and No.7 soil samples which were numbered 1-3-6, 4-4-2, 6-2-27 and 7-2-13 respectively, accounted for 5.50% in separated strains. [Conclusion] This study laid certain foundation for biological control of alfalfa root rot disease.[Objective] Actinomycetes with high antagonistic effects on alfalfa root rot pathogen was isolated from 10 soil samples in Chifeng Inner Mongolia. [Method] 91 actinomyces were separated from 10 soil samples in Chifeng Inner Mongolia by gradient dilution separation method. In duel culture tests, all isolates were tested for their antagonism by using 3 strains including Fusarium solani, F. oxysoporum, F. avenaceum of alfalfa root rot pathogenic bacteria as indicator strains. [Result] 5 strains with strong antagonistic effect on tested alfalfa root rot pathogen were obtained from No.1, No.4, No.6 and No.7 soil samples which were numbered 1-3-6, 4-4-2, 6-2-27 and 7-2-13 respectively, accounted for 5.50% in separated strains. [Conclusion] This study laid certain foundation for biological control of alfalfa root rot disease.

关 键 词：ACTINOMYCES Antagonistic effect ALFALFA Root rot 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]