Biological Characteristics and Pathogenicity of Verticillium lecanii Isolated from Naturally Died Boettcherisca peregrine  

罹病棕尾别麻蝇体上蜡蚧轮枝菌的生物学特性与致病性研究(英文)

作　　者：王凌超[1] 高熹[1] 王松田[1] 刘小文[1] 叶敏[1] 吴国星[1] 

机构地区：[1]云南农业大学植物保护学院,云南昆明650201

出　　处：《Plant Diseases and Pests》2010年第2期28-31,40,共5页植物病虫害研究（英文版）

基　　金：Supported by Yunnan Provincial Department of Education,Fund Projects of Young Teachers(06y15SB);Yunnan Science and Technology Project(2006NG16)~~

摘　　要：[Objective] In order to find the pathogenic microorganisms suitable for biological control of filth flies, the pathogenic microorganism was isolated from the dead fly, Boettcherisca peregrine. [Method] The conidia and mycelia were observed by optical microscope. The pathogenic microorganism was identified on the basis of its culture characters and the optical morphologies of the conidia and mycelia, and its biological characteristics and pathogenicity were preliminarily studied. [Result] The pathogenic microorganism isolated from the dead fly was a new strain of Verticillium lecanii. The new strain of V. lecanii was numbered as KMZW-1. The colonies of V. lecanii KMZW-1 grew fastest on potato dextrose agar medium （PDA） at 29 ℃ and pH 6.0. The LC50 of its spore suspension to the adults of B. peregrine, Lucilia sericata, Musca domestic, Piophila casei and Drosophila melanogaster were 9.50×10^5, 4.58×10^7, 4.06×10^7, 4.10×10^3 and 1.05×10^7 conidia/ml, respectively. The LT50 were 6.86, 8.17, 8.16, 8.12 and 3.22 d, respectively. [Conclusion] V. lecanii KMZW-1 is an active pathogenic microorganism to control the adults of five fly species.[Objective] In order to find the pathogenic microorganisms suitable for biological control of filth flies, the pathogenic microorganism was isolated from the dead fly, Boettcherisca peregrine. [Method] The conidia and mycelia were observed by optical microscope. The pathogenic microorganism was identified on the basis of its culture characters and the optical morphologies of the conidia and mycelia, and its biological characteristics and pathogenicity were preliminarily studied. [Result] The pathogenic microorganism isolated from the dead fly was a new strain of Verticillium lecanii. The new strain of V. lecanii was numbered as KMZW-1. The colonies of V. lecanii KMZW-1 grew fastest on potato dextrose agar medium （PDA） at 29 ℃ and pH 6.0. The LC50 of its spore suspension to the adults of B. peregrine, Lucilia sericata, Musca domestic, Piophila casei and Drosophila melanogaster were 9.50×10^5, 4.58×10^7, 4.06×10^7, 4.10×10^3 and 1.05×10^7 conidia/ml, respectively. The LT50 were 6.86, 8.17, 8.16, 8.12 and 3.22 d, respectively. [Conclusion] V. lecanii KMZW-1 is an active pathogenic microorganism to control the adults of five fly species.

关 键 词：Boettcherisca peregrine Verticillium lecanii Biological characteristic PATHOGENICITY 

分 类 号：S476[农业科学—农业昆虫与害虫防治]