Cricket Grb-AST_7 Gene Concatemer Constructed by "Self Template-primer" PCR  

“自身模板引物”PCR法构建蟋蟀神经肽Grb-AST_7基因串联体(英文)

作　　者：查笑君[1,2] 马伯军[1] 潘建伟[1] 黄俊生[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《Plant Diseases and Pests》2010年第2期45-48,共4页植物病虫害研究（英文版）

基　　金：Supported by National Science and Technology Support Projects(No.2007BAD48B02);Agriculture Science and Technology Achievement Transformation Project(No.2007GB23260410);Basic Research Operating Expenses in Central Public Research Institutes(2007HZS1J007)~~

摘　　要：[Objective] Grb-AST7 concatemer including 17 copies of Grb-AST7 was constructed by ＂self template-primer＂ PCR. [Method] Two primers of which were synthesized based on the amino acid sequence of Gryllus bimaculatus’ Grb-AST7. [Result] Through splicing, a length of 570 bp, containing 17 copies of the Grb-AST7 gene repeats were obtained. Appropriate primer splicing conditions were as follows：splice time 20 min, 25 μl PCR system, containing 2 μl template. [Conclusion] The results laid foundation for the future studies on Grb-AST7 gene expression and bio-activity analysis.[Objective] Grb-AST7 concatemer including 17 copies of Grb-AST7 was constructed by ＂self template-primer＂ PCR. [Method] Two primers of which were synthesized based on the amino acid sequence of Gryllus bimaculatus’ Grb-AST7. [Result] Through splicing, a length of 570 bp, containing 17 copies of the Grb-AST7 gene repeats were obtained. Appropriate primer splicing conditions were as follows：splice time 20 min, 25 μl PCR system, containing 2 μl template. [Conclusion] The results laid foundation for the future studies on Grb-AST7 gene expression and bio-activity analysis.

关 键 词：Grb-AST7 ＂Self template-primer＂ PCR Concatemer 

分 类 号：S186[农业科学—农业基础科学]