Screening of Antagonistic Actinomyces against Soft Rot of Amorphophallus konjac and Its Antibacterial Activity  

魔芋软腐病拮抗放线菌的筛选及其抑菌活性研究(英文)

作　　者：叶晶龙[1] 潘虹[1] 廖甜甜[1] 李金鞠[1] 刘海军[1] 王钧[2] 乐超银[1] 

机构地区：[1]三峡大学生物技术研究中心,湖北宜昌443002 [2]三峡大学校医院,湖北宜昌443002

出　　处：《Plant Diseases and Pests》2010年第5期30-33,共4页植物病虫害研究（英文版）

基　　金：Supported by Key Laboratory Fund in Hubei Province(2008NP11);Science and Technology Research and Development Project in Yichang City(A09302-17)~~

摘　　要：[Objective] Antagonistic actinomyces of Amorphophallus konjac soft rot were isolated from the soil,the antibacterial activity of the fermentation was detected,and its active components were separated.[Method] The antagonistic actinomycetes were screened using plate confrontation culture method.The SJK18 antagonistic strain with strong activity was conducted single-factor tests using different carbon sources,nitrogen sources,temperatures and pH value as the culture condition,and the antibacterial activities of fermentation liquid was determined,respectively.The fermentation liquid of antagonistic strain was extracted with alcohol and acetic ester,and their antibacterial activities were determined.[Result] A total of 112 strains of actinomycetes were obtained,and 11 strains had antibacterial activity.The antibacterial activity of SJK18 strain was the strongest in the single-factor test,when the corn powder was adopted as the carbon sources,KNO3 was the nitrogen source,the temperature was 30 ℃ and the pH was 6.0,the appropriate pH value for the activity was 5.5-10.0.The diameters of inhibition zone of alcohol extract and raffinate of ethyl acetate were 17.0 and 20.0 mm,respectively,while the extraction of ethyl acetate had no antibacterial activity.[Conclusion] A strain of actinomyces with strong antibacterial activity against soft rot of A.konjac was obtained.[Objective] Antagonistic actinomyces of Amorphophallus konjac soft rot were isolated from the soil,the antibacterial activity of the fermentation was detected,and its active components were separated.[Method] The antagonistic actinomycetes were screened using plate confrontation culture method.The SJK18 antagonistic strain with strong activity was conducted single-factor tests using different carbon sources,nitrogen sources,temperatures and pH value as the culture condition,and the antibacterial activities of fermentation liquid was determined,respectively.The fermentation liquid of antagonistic strain was extracted with alcohol and acetic ester,and their antibacterial activities were determined.[Result] A total of 112 strains of actinomycetes were obtained,and 11 strains had antibacterial activity.The antibacterial activity of SJK18 strain was the strongest in the single-factor test,when the corn powder was adopted as the carbon sources,KNO3 was the nitrogen source,the temperature was 30 ℃ and the pH was 6.0,the appropriate pH value for the activity was 5.5-10.0.The diameters of inhibition zone of alcohol extract and raffinate of ethyl acetate were 17.0 and 20.0 mm,respectively,while the extraction of ethyl acetate had no antibacterial activity.[Conclusion] A strain of actinomyces with strong antibacterial activity against soft rot of A.konjac was obtained.

关 键 词：Soft rot of A.konjac Antagonistic actinomycetes Confrontation culture Antibacterial activity 

分 类 号：S436.32[农业科学—农业昆虫与害虫防治]