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作 者:朱文奇[1] 李慧芳[1] 吴蓉蓉[2] 宋卫涛[1] 徐文娟[1] 于吉英 肖小君[2,3] 陈宽维[1]
机构地区:[1]中国农业科学院家禽研究所,江苏扬州225003 [2]扬州大学动物科学与技术学院,江苏扬州225003 [3]海南罗牛山文昌鸡育种有限公司,海南海口570000
出 处:《Animal Husbandry and Feed Science》2009年第2期5-7,12,共4页动物与饲料科学(英文版)
基 金:国家科技支撑计划(2008BADB2B07);国家高技术研究发展计划(2006AA10Z1D8);农业行业专项研究计划(nyhyzx07-039);现代农业产业技术体系建设专项资金~~
摘 要:[ Objective] The paper presents the polymorphism of neuropeptide Y gene (NPY) and its relationship with traits at first laying in Wen- chang chicken in order to provide information for breeding of high-yielding Wenchang chicken. [Method] The polymorphism of NPYgene at Dra I site was detected by PCR-RFLP, and the genotypes and the genetic stability were determined. The correlation between the polymorphism and traits at first laying (age, body weight and egg weight) was also analyzed. [Result] There were three kinds of genotype of NPYgene at Dra I site in Wenchang chicken, designated as AA, AB and BB; the distribution of these genotypes was consistent with the Hardy -Weinberg law; age and egg weight were not significantly different among the three genotypes ( P 〉0.05), and body weight was extremely significantly different ( P 〈0.01 ). [ Conclusion] The NPYgene of Wenchang chicken is in Hardy -Weinberg equilibrium at Dra I site, and its genetic polymorphism affects body weight at first laying.[ Objective] The paper presents the polymorphism of neuropeptide Y gene (NPY) and its relationship with traits at first laying in Wen- chang chicken in order to provide information for breeding of high-yielding Wenchang chicken. [Method] The polymorphism of NPYgene at Dra I site was detected by PCR-RFLP, and the genotypes and the genetic stability were determined. The correlation between the polymorphism and traits at first laying (age, body weight and egg weight) was also analyzed. [Result] There were three kinds of genotype of NPYgene at Dra I site in Wenchang chicken, designated as AA, AB and BB; the distribution of these genotypes was consistent with the Hardy -Weinberg law; age and egg weight were not significantly different among the three genotypes ( P 〉0.05), and body weight was extremely significantly different ( P 〈0.01 ). [ Conclusion] The NPYgene of Wenchang chicken is in Hardy -Weinberg equilibrium at Dra I site, and its genetic polymorphism affects body weight at first laying.
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