Single Nucleotide Polymorphism Analysis on 5' Regulatory Region of Goose Insulin-like Growth FactorⅠGene  

鹅IGF-Ⅰ基因5'调控区单核苷酸多态性分析(英文)

作　　者：杜晓东[1] 张绍胜 姜润深[1] 夏生林 耿照玉[1] 袁绍友 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]芜湖动物疾病预防与控制中心,安徽芜湖241000 [3]安徽六安皖西白鹅原种场,安徽六安237005

出　　处：《Animal Husbandry and Feed Science》2009年第2期13-14,42,共3页动物与饲料科学（英文版）

摘　　要：[ Objedive] This study was aimed to determine the single nucleotide polymorphisms （SNPs） of IGF-I gene in two breeds, Wanxi white goose and Langde goose. [ Method] Two pair of primers was designed based on chicken and porcine genomic sequence to amplify the 5＇ regulatory region of IGF-I, and the sequence was determined and analyzed. [ Result] A total of four SNPs were identified in this region by PCR-SSCP meth- od, that is, A to T at 26 nt, A to G at 215 nt, A to G at 314 nt, and A to T at 325 nt. [ Conclusioa] The two breeds ,wanxi white geese and Langde geese, agree with Hardy-weinberg equilibrium with respect to these SNPS.[ Objedive] This study was aimed to determine the single nucleotide polymorphisms （SNPs） of IGF-I gene in two breeds, Wanxi white goose and Langde goose. [ Method] Two pair of primers was designed based on chicken and porcine genomic sequence to amplify the 5＇ regulatory region of IGF-I, and the sequence was determined and analyzed. [ Result] A total of four SNPs were identified in this region by PCR-SSCP meth- od, that is, A to T at 26 nt, A to G at 215 nt, A to G at 314 nt, and A to T at 325 nt. [ Conclusioa] The two breeds ,wanxi white geese and Langde geese, agree with Hardy-weinberg equilibrium with respect to these SNPS.

关 键 词：GOOSE Insulin-like growth factorlgene Single nucleotide polymorphisms 

分 类 号：S835[农业科学—畜牧学]