Proteomic-Level Responses of Early-Weaned Piglets to Dietary Arginine Supplementation  

精氨酸调控仔猪断奶应激的蛋白质双向电泳分析(英文)

作　　者：王文策[1] 耿梅梅[1] 李铁军[1] 孔祥峰[1] 褚武英[1] 顾婉婷[1] 潘杰[1] 傅德志[1] 冯泽猛[1] 杨焕胜[1] 黄瑞林[1] 印遇龙[1] 

机构地区：[1]湖南省畜禽健康养殖工程技术研究中心亚热带农业生态过程重点实验室中国科学院亚热带农业生态研究所,湖南长沙410125

出　　处：《Animal Husbandry and Feed Science》2009年第4期29-33,共5页动物与饲料科学（英文版）

基　　金：Supported by grants from the Knowledge Innovation Project of Chinese Academy of Sciences ( KSCX2-YW-N-051 and SW-323);NSFC(30901040, 30901041, 30928018, 30828025, 30700581, and 30771558 );National Basic Research Program of China(2009CB118800);National 863 Project ( 2008AA10Z316);National Scientific and Technological Supporting Project(2007BAQ01047 and 2006BAD12B07)~~

摘　　要：Dietary arginine supplementation enhances the immune status and protein synthesis in early-weaned pigs. However, the underlying mechanisms remain largely unknown. To investigate how arginine affects the expression of key proteins that regulate growth and nutrient transport of jejunum, a total of 12 healthy piglets （21 day-old, similar body weight, Landrace x Yorkshire） delivered by four sows were randomly divided into two groups. Piglets in the test group were reared with feed supplemented L-arginine at a concentration of 6.0 g/kg, while piglets in the control group were fed with feed supplemented L-alanine at a concentration of 12.3 g/kg （isonitrogenous control）. After 7 d, jejunum mucosae was collected and analyzed with the 2-D PAGE MS technology. Compared with the control pigs, arginine decreased the levels of proteins that regulate the protein syn- thesis, intermediary metabolism and tissue growth （ similar to anterior gradient 2 homolog, cyclophilin_ABHJike, hypothetical protein FLJ39502 and tetratrioopeptide repeat domain 16, similar to KIAA0156, mitechondrial ATP synthase, hydrion transporting F1 complex, beta subunit and alpha-tu- bulin ubiquitous isoform 19, prolyl 4-hydroxylase, beta subunit precursor）. In addition, arginine increased the levels of proteins that are involved in proteolysis and immune response （ PGAM1, T cell receptor beta variable 20, membrane steroid binding protein, similar to myomesin-1, and chain A, structure of pig muscle Pgk complexed with MgATP）. Therefore, arginine influences the immune response and protein synthesis mechanisms as well as improves eady weaned stress syndrome of piglets.Dietary arginine supplementation enhances the immune status and protein synthesis in early-weaned pigs. However, the underlying mechanisms remain largely unknown. To investigate how arginine affects the expression of key proteins that regulate growth and nutrient transport of jejunum, a total of 12 healthy piglets （21 day-old, similar body weight, Landrace x Yorkshire） delivered by four sows were randomly divided into two groups. Piglets in the test group were reared with feed supplemented L-arginine at a concentration of 6.0 g/kg, while piglets in the control group were fed with feed supplemented L-alanine at a concentration of 12.3 g/kg （isonitrogenous control）. After 7 d, jejunum mucosae was collected and analyzed with the 2-D PAGE MS technology. Compared with the control pigs, arginine decreased the levels of proteins that regulate the protein syn- thesis, intermediary metabolism and tissue growth （ similar to anterior gradient 2 homolog, cyclophilin_ABHJike, hypothetical protein FLJ39502 and tetratrioopeptide repeat domain 16, similar to KIAA0156, mitechondrial ATP synthase, hydrion transporting F1 complex, beta subunit and alpha-tu- bulin ubiquitous isoform 19, prolyl 4-hydroxylase, beta subunit precursor）. In addition, arginine increased the levels of proteins that are involved in proteolysis and immune response （ PGAM1, T cell receptor beta variable 20, membrane steroid binding protein, similar to myomesin-1, and chain A, structure of pig muscle Pgk complexed with MgATP）. Therefore, arginine influences the immune response and protein synthesis mechanisms as well as improves eady weaned stress syndrome of piglets.

关 键 词：ARGININE Early-weaning PIGLETS PROTEOME 

分 类 号：S828[农业科学—畜牧学]