Cloning and Sequence Analysis of Porcine IRGC Gene  

作　　者：WU Hua-yu QIAO Mu LI Liang-hua GUO Wan-zheng PENG Xian-wen SUN Hua HUANG Jing-shu MEI Shu-qi 

机构地区：[1]Institute of Animal Science and Veterinary Medicine, Hubei Academy of Agricultural Science, Wuhan 430064, China [2]College of Animal Science and Technology, Huazhong Agricultural Univerisity, Wuhan 430070, China

出　　处：《Animal Husbandry and Feed Science》2009年第6期10-11,15,共3页动物与饲料科学（英文版）

基　　金：supported by National Key Technology R&D Program (2006BAD01A08,2008BADB2B02);Program from Hubei Innovation Center of Agricultural Science and Technology(2007-602-001-03) ;Special Fund for Constructing National Technology System for Pig Industry(NYCYTX-009)

摘　　要：[ Objective] This study was to analyze porcine IRGC gene, so as to lay the foundation of further revealing its function. [ Method] IRGC gene was cloned by using EST information together with sequencing, then aligned with the IRGC sequences of human, cattle, dog and orangutan for obtaining their similarities. [Result] Porcine IRGC cDNA obtained in the present study was 1 558 bp in length（GenBank accession number EU703776）, which shared high similarity with human （86%）, cattle （90%）, dog （91%）, orangutan （84%）. Sequence analysis shows that porcine IRGC gene encodes 464 amino acid residues which share high homologies with the counterparts from human （90%）, cattle （93%）, dog （95%）, orangutan （90%）. [Condusion] Cloning and sequence analysis of porcine IRGC gene would be helpful for studying its functions.[ Objective] This study was to analyze porcine IRGC gene, so as to lay the foundation of further revealing its function. [ Method] IRGC gene was cloned by using EST information together with sequencing, then aligned with the IRGC sequences of human, cattle, dog and orangutan for obtaining their similarities. [Result] Porcine IRGC cDNA obtained in the present study was 1 558 bp in length（GenBank accession number EU703776）, which shared high similarity with human （86%）, cattle （90%）, dog （91%）, orangutan （84%）. Sequence analysis shows that porcine IRGC gene encodes 464 amino acid residues which share high homologies with the counterparts from human （90%）, cattle （93%）, dog （95%）, orangutan （90%）. [Condusion] Cloning and sequence analysis of porcine IRGC gene would be helpful for studying its functions.

关 键 词：PIG IRGC gene CLONE Sequence analysis 

分 类 号：Q785[生物学—分子生物学] Q78