耐铅微生物筛选及其铅去除能力的初步研究  

Screening of Lead-Tolerant Microbes and Preliminary Study on Their Removal Capability to Lead

作　　者：杨亮[1] 郝瑞霞[1] 吴沣[1] 肖育雄[1] 

机构地区：[1]北京大学地球与空间科学学院造山带与地壳演化重点实验室,北京100871

出　　处：《北京大学学报（自然科学版）》2012年第6期965-970,共6页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家重点基础研究发展计划(2007CB815601)资助

摘　　要：从铅锌尾矿厂周围土壤样品中分离出4株耐铅菌。4株耐铅菌均能在Pb2＋浓度范围为100~1000 mg/L的培养液中生长。在pH 6,25℃,160 r/min及初始Pb2＋浓度为600 mg/L时,菌株Pb-X-1,Pb-Z-3和Pb-Z-4的Pb2＋去除率分别为78.31%,77.8%和87%。菌株Pb-X-2在初始Pb2＋浓度为100~400 mg/L时,去除率均高于95%。16S rRDA基因序列分析表明,Pb-X-2属于嗜麦芽寡养单胞菌。菌株Pb-X-2对溶液中铅离子的去除机理为细胞表面吸附与胞内富集。Four lead-tolerant strains were isolated from lead-contaminated soil around the lead-zinc ore deposit. All strains could grow in nutrient solution containing 100-1000 mg/L Pb（II）. The removal rates of Pb（II） by Pb-X-1, Pb-Z-3 and Pb-Z-4 were 78.31%, 77.8% and 87% respectively in the following conditions： pH was 6, 25℃, 160 r/min, initial pb2＋eoncentration was 600 mg/L. The Pb（II）removal rate by Pb-X-2 was higher than 95%, when initial Pb（II）concentration was 100-400 mg/L. The result of 16S rRNA sequencing indicates that Pb-X-2 belonged to Stenotrophomonas maltophilia. The removal mechanism of Pb（II） removal by Pb-X-2 was mainly cellular surface adsorption and intracellular enrichment.

关 键 词：铅 去除 嗜麦芽寡养单胞菌 

分 类 号：X53[环境科学与工程—环境工程] X172