12个草地早熟禾品种愈伤组织诱导体系的研究  

A study on the callus induction system in 12 Poa pratensis L.varieties

作　　者：代亮[1] 柳玉霞[1] 刘洁[1] 李昊阳[1] 王晓桐[1] 徐吉臣[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室,北京100083

出　　处：《云南大学学报（自然科学版）》2012年第6期722-730,共9页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(31128016);北京市自然科学基金资助项目(5112015)

摘　　要：以生产中常见的12种草地早熟禾(Poa pratensis L.)成熟种子为外植体,对其愈伤组织诱导及愈伤组织增殖生长体系进行了优化试验.结果表明,在愈伤组织诱导试验中,单纯使用2,4-D时,不同品种愈伤组织诱导率在60%～93%之间,2,4-D质量浓度3 mg/L时12个早熟禾品种的愈伤组织诱导率均值最高,达到86%;在2,4-D基础上添加少量6-BA(0.1～0.2 mg/L)后,不同品种愈伤组织诱导率在30%～92%之间,12个品种的愈伤组织诱导率均值有所降低(3.1%～25.4%).在愈伤组织增殖试验中,单纯使用2,4-D时,不同品种愈伤组织增殖率在38%～164%之间,12个早熟禾品种的愈伤组织增殖率均值在3 mg/L 2,4-D时最高,达到81%;在3 mg/L 2,4-D基础上施加少量的6-BA(0.05～0.2 mg/L)后,愈伤组织增殖率均值有所降低,但存在品种的差异性.研究结果还显示,热激处理对部分草地早熟禾愈伤组织诱导及增殖有一定的促进作用.12 Kentucky bluegrass varieties （Poa pratensis L. ） were randomly selected as the materials for the test of callus induction and proliferation, taking mature seeds as explants. Varieties showed different rates in forming callus. With 2,4 - D only,it varied from 60% to 93%. With 2,4 - D at 3mg/L,the average rate of 12 varieties reached to 86%. By adding some 6 - BA（0.1--0.2 mg/L）additionally, the rates changed from 30% to 92% ,but the average rate was reduced a little. In the callus proliferation test, the proliferation rates were in a range of 38%--164% with only 2,4 -D. The average rate reaches to 81% with the treatment of 3 mg,/k 2,4 - D. Additional 6 - BA at 0.05---0.2 mg/L reduced the average proliferation rate, and the reduction was changing by different varieties. The results also showed that the heat shock increased the rate of callus induction and prolif- eration for some Kentucky bluegrass varieties.

关 键 词：草地早熟禾 组织培养 愈伤组织诱导 增殖率 

分 类 号：Q945.39[生物学—植物学]