心痛方对心肌缺血大鼠黏附分子表达的干预作用  

INTERVENTION EFFCT OF XINTONG PRESCRIPTION ON ADHESION MOLECULES EXPRESSION IN ISCHEMIC MYOCARDIUM OF RATS

作　　者：范金茹[1] 匡秀青[1] 姜益宏[1] 陈彤[1] 杨金怡[1] 

机构地区：[1]湖南中医药大学第一附属医院内科,长沙410007

出　　处：《中国中医药科技》2012年第6期494-495,480,共2页Chinese Journal of Traditional Medical Science and Technology

摘　　要：目的:探讨心痛方对心肌缺血大鼠心肌梗死面积的影响及黏附分子ICAM-1、VCAM-1表达的干预作用。方法:80只SD大鼠,随机分成假手术组、模型组、心痛方组、欣康对照组,采用结扎法制备心肌缺血模型,NBT染色法测定心肌梗死面积,免疫组化法检测黏附分子在缺血心肌的表达。结果:心痛方和欣康均能缩小心肌梗死面积,心痛方呈现出优于欣康的趋势。与假手术组相比,心痛方组、欣康组、模型组缺血心肌ICAM-1、VCAM-1表达的AOD均明显增高(P<0.01);与模型组相比,心痛方组、欣康组ICAM-1、VCAM-1表达的AOD均明显降低(P<0.01);心痛方与欣康在抑制ICAM-1表达方面作用相近,在抑制VCAM-1表达上较欣康略显优势。结论:心痛方能缩小大鼠心肌梗死面积,抑制缺血心肌ICAM-1、VCAM-1的表达。Objective:To explore the intervention effect of xintong prescription on myocardial infarction area and adhesion molecules expression in myocardial ischemia rats.Methods:80 rats were divided into four groups,sham operation,model,xinkang and xintong prescription groups,the models with myocardial ischemia were built by ligation method,each group was given the saline or corresponding medicine for 7 days,myocardial infarction area was measured by NBT stainning,adhesion molecules ICAM-1 and VCAM-1 expression in ischemic myocardium of rats was determined by immunohistochemistry method.Results:To compare with model group,the myocardial infarction area of rats in xintong prescription group and positive group(xinkang) was obviously reduced.To compare with sham operation group,the ICAM-1 and VCAM-1 expression in ischemic myocardium of rats increased significantly,those were decreased obviously in xintong prescription and xinkang groups,there was no significant difference between xintong prescription and xinkang groups.Conclusion:Xintong prescription can lessen myocardial infarction area,inhibit ICAM-1 and VCAM-1 expression in ischemic myocardium.

关 键 词：心肌缺血/中医药疗法 @心痛方/治疗应用 黏附分子 大鼠 

分 类 号：R285.5[医药卫生—中药学]