大鼠脊髓损伤后CBS和BBB评分比较研究  

Comparison Study on BBB Test with Combined Behavioral Score after Spinal Cord Injury in Rats

作　　者：殷铭[1] 徐又佳[2] 

机构地区：[1]无锡市中医医院骨伤科,江苏无锡214001 [2]苏州大学附属第二医院科教处,江苏苏州215004

出　　处：《中国血液流变学杂志》2012年第3期398-400,505,共4页Chinese Journal of Hemorheology

摘　　要：目的比较两种常用的标准评分方法评价大鼠胸段脊髓损伤后以及恢复过程的后肢功能的优劣性。方法选用20只体重为240～260g的成年雌性Wistar大鼠，随机分成2组，分别为轻度损伤组和重度损伤组，均采用Allen打击法制成急性脊髓损伤模型。采用双盲设计，3名经过训练的硕士研究生都分别以两种评分方法对大鼠后肢功能进行评价，并进行统计分析。结果两种评分方法与损伤程度都有相关性（P〈0．05）。在组间比较中，CBS评分从第2天起体现差异性（P〈0．05），BBB评分从6h起体现差异性；在组内比较中，CBS评分和BBB评分在轻度损伤组相邻的时间点均体现有差异性（P〈0．05），在重度损伤组组内比较中，CBS评分敏感性与轻度组相当；而BBB评分到第4天才出现差异性（P〈0．05）。结论在不同损伤组组间的比较中BBB评分方法具有优势，可作为急性脊髓损伤模型对比研究的标准评分法。Objective To compare the combined behavioral score with BBB after the rat spinal cord was injured.Methods Twenty Wistar rats weighted 240 - 260g were divided into two groups randomly, one was mild group and the other was serious group;acute spinal cord injury models were made using Allen＇s method,and then compared the two behavior scores according to the extent of injury by three trained masters in double blind. Results Two behavior scores all had dependability in hind limb locomotor function according to the extent of injury.The combined behavioral score had statistical significance（P 〈 0.05） from the next day, and the BBB score had statistical significance（P 〈 0.05） 6 hours later in interclass contrast.The combined behavioral score and the BBB score both had statistical significance（P 〈 0.05） in mild intragroup contrast.In the serious group,the combined behavioral score had the same sensitivity as the mild group,but were better than combined behavioral score in dependability and truth,but the BBB score had statistical significance（P 〈 0.05） four days later.Conclusion The BBB scale is ascendant in interclass comparison.It could be a standard contrast investigative test for spinal cord injury in rats.

关 键 词：脊髓损伤 行为评分 评分标准 大鼠 

分 类 号：R651.2[医药卫生—外科学]