福建省志贺菌PFGE分型研究  

Molecular typing of Shigella strains using PAGE in Fujian

作　　者：杨劲松[1] 李海丹[1] 陈爱平[1] 罗朝晨[1] 郑金凤[1] 严延生[1] 

机构地区：[1]福建省疾病预防控制中心,福建福州350001

出　　处：《中国预防医学杂志》2012年第11期811-815,共5页Chinese Preventive Medicine

基　　金：国家科技重大专项"传染病监测技术平台"项目(2009ZX10004-210);福建省卫生厅青年基金课题资助计划项目(2010-1-23)

摘　　要：目的了解福建省志贺菌脉冲场凝胶电泳(PFGE)分子分型情况,描述基因群的流行及分布特征,探讨优势血清群的变迁规律。方法收集2005-2010年志贺菌96株,分离自临床患者。选择NotⅠ进行酶切,H9812作为脉冲场凝胶分子量标准。采用脉冲场凝胶电泳技术分析电泳酶切指纹图谱,运用BioNumer-ics软件进行聚类分析。结果按照100%的相似度可将34株福氏志贺菌酶切图谱分为27种PFGE型别;将62株宋内志贺菌酶切图谱分为43种PFGE型别。根据TENOVER原则,福氏志贺菌有2个优势基因群G1-G2,宋内志贺菌有4个优势基因群GI-GIV。优势基因群集中分布于7～10月,其他PFGE型别分布比较分散,无明显时间和地区的聚集性。结论福建省志贺菌分子分型呈现多样性,宋内和F4c的某些基因群可能逐渐演变为福建省优势且稳定的志贺菌流行菌株。Objective To study the molecular typing of Shigella in Fujian by pulsed field gel electrophoresis （PFGE）, and to describe the epidemiological features of gene groups. Methods 96 strains of Shigella were isolated from patients and were analyzed by PAGE after digestion with Not I enzyme. H9812 was used as mo lecular weight marker and BioNumerics for cluster analysis. Results Based on 100% similarity, the finger printings of 34 Shigella flexneri and 62 Shigella sonnei were divided into 27 and 43 PFGE types. According to ＂ TENOVER principle＂, there were 2 dominant gene groups （G1-G2） of Shigella flexneri and 4 prevalent gene groups （GI-GIV） of Shigella sonnei. The dominant gene groups usually appeared from July to October, while other types could appear at any time without regional aggregation. Conclusions The diversity of molecular types of Shigella in Fujian is obvious, and some prevalent gene groups of Shigella flexneri and Shigella sonnei may gradually evolve to predominant and stable epidemic strains.

关 键 词：志贺菌 脉冲场凝胶电泳(PFGE) 分子分型 基因群 

分 类 号：R378[医药卫生—病原生物学]