探讨阿司匹林对百草枯中毒大鼠肝肾功能的保护作用  

Discuss Aspirin's Protective Effects on Paraquat Poisoning Rat Liver and Kidney Function

作　　者：刘洪英[1] 宋津晓[1] 王梓凌[1] 董琦[1] 齐惠军[1] 斯重阳[1] 

机构地区：[1]河北省人民医院急诊科,石家庄050051

出　　处：《中国医药导刊》2012年第10期1808-1810,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:探讨阿司匹林对百草枯(PQ)中毒大鼠肝肾功能的保护作用,以进一步探讨阿司匹林对百草枯中毒大鼠肝功能的保护作用。方法:取90只健康SD大鼠作为研究对象,随机分为PQ单纯染毒组、赖氨酸阿司匹林治疗组及生理盐水对照组,每组30只,大鼠以单纯染毒组予20%PQ 50 mg/kg一次性灌胃,3 h后予1 ml生理盐水腹腔注射;治疗组予20%PQ 50 mg/kg一次性灌胃染毒,3 h后予200mg/kg赖氨酸阿司匹林一次性腹腔注射;对照组予生理盐水2 ml一次性灌胃,3 h后予1 ml生理盐水腹腔注射,大鼠在染毒后在第1、3、7和14 d各取一组,用药物腹腔注射麻醉后分批处死大鼠,钝性的分离出来腹部主动脉后,用一次性的5ml的注射器进行腹主动脉采血,测定大鼠的血清和肾组织中的血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、肌酐(Cr)和血尿素氮(BUN),右肾用10%的福尔马林溶液进行侵泡,进行病理分析。结果:PQ染毒后第3、7和14 d染毒组血清中ALT、AST、Cr和BUN的水平明显高于治疗组和对照组(P<0.05),第1、3和7 d治疗组明显高于对照组(P<0.05),第1 d染毒组血清中ALT、AST、Cr和BUN的水平明显高于对照组(P<0.05),与治疗组相比,差异没有统计学意义(P>0.05),第14 d治疗组与对照组比较,差异没有统计学意义(P>0.05)。染毒组病理改变有充血水肿、炎症细胞浸润、变性坏死及结构紊乱等;治疗组改变明显减轻,且无结构紊乱;对照组结构清晰,未见充血水肿、炎症细胞浸润及坏死等病理表现。结论:阿司匹林对百草枯中毒大鼠的肝肾功能有一定的保护作用。Objective：To discuss aspirin’s protective effects on paraquat poisoning rat liver and kidney function.Methods：Chose 90 healthy SD rats as study objectives,divided them into PQ simple infection group,lysine aspirin treatment group and normal saline control group randomly,each group has 30 cases,gave 20%PQ 50 mg/kg of intragastric infusion to rats in simple infection group,and gave 1ml normal saline to them after 3h;Gave 20%PQ 50 mg/kg of intragastric infusion to rats in treatment group and gave 200 mg/kg of lysine aspirin intraperitoneal injection to them after 3h;While gave 2ml of normal saline to rats in control group and gave 1ml of normal saline intraperitoneal injection to them.After 1,3,7 and 14 days of contamination,used drug intraperitoneal injection of anesthesia to kill the rats,after the separation of blunt abdominal aorta out,adopted disposable 5ml injector to proceed abdominal aorta draw blood,tested rats’ blood serum,serum glutamic pyruvic transaminase,glutamic-oxalacetic transaminease,creatinine and blood urea nitrogen,used 10% of formalin solution to soak their right kidney,and analysed their pathologies.Results：PQ infection group’s rats’ ALT、AST、Cr and BUN level in blood serum were obviously higher than treatment group’s and control group’s（P0.05） after 3,7 and 14days,and treatment group’s were evidently higher than control group’s after 1,3 and 7 days（P0.05）,the infection group’s ALT、AST、Cr and BUN level in blood serum at the first day were obviously higher than control group’s（P0.05）,compared with treatment group,the difference has no statistics meaning（P0.05）,compared treatment group and control group in the 14th day,the difference also has no statistics meaning（P0.05）.Infection group’s pathology changes appeared congestion edema,inflammation cells infiltration,degeneration necrosis and structure disorder,etc;Treatment group’s changes reduced significantly and did not appear structure disorder;Control group’s structure was clear,and di

关 键 词：阿司匹林 百草枯 肝功能作用 中毒 肝保护 

分 类 号：R595.4[医药卫生—内科学]