高效液相色谱法测定参芪补气片中黄芪甲苷的含量  

Determination of Astragaloside Ⅳ in Shenqi Buqi Tablets by HPLC

作　　者：颜海弟 谭丽容[2] 陈越 何景进[2] 李楚文[2] 吴典伟[2] 苏子仁[2] 

机构地区：[1]广东省湛江市第二人民医院,广东湛江524008 [2]广州中医药大学,广州510006

出　　处：《中国医院用药评价与分析》2012年第10期924-926,共3页Evaluation and Analysis of Drug-use in Hospitals of China

摘　　要：目的:建立高效液相色谱(HPLC)法测定参芪补气片中黄芪甲苷的含量。方法:以吡啶∶苯甲酰氯(2∶1)为衍生化试剂,对黄芪甲苷进行苯甲酰化,采用HPLC法在270 nm波长处测定参芪补气片中黄芪甲苷的含量。结果:黄芪甲苷进样量在0.078～0.975μg范围内线性关系良好(r=0.999 9),重复性好(RSD=0.82%),回收率平均值为99.51%～100.76%(RSD=0.29%～1.11%)。结论:该法样品前处理简单、专属性强、准确度高、灵敏度高、重现性好,可以作为控制参芪补气片中黄芪甲苷质量的方法。OBJECTIVE： To establish HPLC determination of astragaloside Ⅳ in Shenqi Buqi tablets.METHODS： By using pyridine-benzoyl chloride（2∶1） as derivatization reagents for benzoylation of astragaloside Ⅳ.The content of Astragaloside Ⅳ in Shenqi Buqi tablets was determined by HPLC at a wavelength of 270 nm.RESUTLS： The linear range of astragaloside Ⅳ was 0.078-0.975 μg（r=0.999 9）.The method had good reproducibility（RSD=0.82%） with average recovery rate of 99.51%-100.76%（RSD=0.29%-1.11%）.CONCLUSION： The method is simple in pretreatment of samples,specific,accurate,sensitive and reproducible,thus it can be used for the quality control of astragaloside Ⅳ in Shenqi Buqi tablets.

关 键 词：高效液相色谱法 参芪补气片 黄芪甲苷 衍生化 

分 类 号：R965[医药卫生—药理学]