周期性机械拉伸对兔角膜成纤维细胞MMP-2/TIMP-2及TGF-β1表达的影响  

Effects of Cyclic Mechanical Stretch on MMP-2/TIMP-2 and TGF-β1 Expression Synthesized by Corneal Fibroblasts

作　　者：王晓君[1] 陈维毅[1] 李晓娜[2] 冯鹏飞[2] 高志鹏[2] 

机构地区：[1]太原理工大学力学学院 [2]太原理工大学应用力学与生物医学工程研究所,太原030024

出　　处：《太原理工大学学报》2012年第6期770-774,共5页Journal of Taiyuan University of Technology

基　　金：国家自然科学基金资助项目(11032008);山西省自然科学基金资助项目(2010021004-2;2008012013-8)

摘　　要：研究了周期性机械拉伸对角膜成纤维细胞外基质中金属蛋白酶-2(Matrix metalloprotei-nase-2,MMP-2)、组织金属蛋白酶抑制剂-2(Tissue inhibitors of metalloproteinase-2,TIMP-2)及转化生长因子-β1(Transforming growth factorβ1,TGF-β1)表达的影响。对正常兔角膜成纤维细胞进行幅度分别为5%、10%及15%的周期性机械拉伸,并分别于拉伸后6h、24h取细胞培养液,采用ELISA方法检测MMP-2、TIMP-2及TGF-β1的含量。当拉伸幅度为5%时,6h、24h后,MMP-2、TIMP-2及TGF-β1的含量与静态对照组相比均无统计学差异;当拉伸幅度为10%时,6h后MMP-2、TIMP-2及TGF-β1的含量与静态对照组相比无统计学差异,24h后MMP-2的含量显著升高(p<0.05);当拉伸幅度为15%时,6h后MMP-2、TIMP-2及TGF-β1的含量与静态对照组相比仍无统计学差异,拉伸24h后MMP-2、TGF-β1含量显著升高,TIMP-2含量显著降低(p<0.05)。结果表明,在体外以一定的幅度周期性拉伸角膜成纤维细胞一定时间后,细胞分泌的MMP-2、TIMP-2及TGF-β1发生显著改变,三者相互作用,共同调节角膜成纤维细胞的生物学行为。To investigate and determine the effect of cyclic mechanical stretch on the expres- sion of Matrix metalloproteinase-2 （MMP-2）, Tissue inhibitors of metalloproteinase-2 （TIMP-2） and Transforming growth factor β1 （TGF-β1）in extracellular matrix remodeled by corneal fibro blasts,corneal fibroblasts were isolated from normal rabbits and subjected to a cyclic stretch regi- men of 5%, 10% and 15% elongation. The concentration of MMP-2, TIMP-2 and TGF-β1 was assayed on aliquots of culture medium by ELISA method after stretched for 6 h and 24 h separate ly. Our results show that; When the amplitude of stretch was 5%, there was no significant difference in MMP-2, TIMP-2 and TGF-β1 among groups at 6 h or 24 h;When the amplitude of stretch was 10%, there was also no significant difference in MMP-2, TIMP-2 and TGF-β1 among groups at 6 h,but level of MMP-2 was significantly（p〈0.05） elevated at 24 h;When the ampli- tude of stretch was 15%, there was also no significant difference in MMP-2, TIMP-2 and TGF-βI among groups at 6 h, but level of MMP-2, TGF-β1 was significantly（p〈0.05） elevated and TIMP-2 decreased at 24 h. It indicates that MMP-2, TIMP-2 and TGF-βI interacted together to mediate the corneal biology behavior when the cornea was subjected to proper mechanical stimula tion.

关 键 词：角膜成纤维细胞 周期性拉伸 金属蛋白酶-2 组织金属蛋白酶抑制剂-2 转化生长因子-β1 细胞培养 

分 类 号：R318.01[医药卫生—生物医学工程]