云南黄连花粉活力和柱头可授性研究  

Study on Pollen Vitality and Stigma Receptivity of Coptis teeta Wall.

作　　者：杨艳娟[1] 梁艳丽[1,2] 杨生超[2] 李龙根[1] 李代勋[1] 谢世清[2] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学,云南省优势中药材规范化种植工程研究中心,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2012年第6期789-793,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家支持计划(2011BAI13B01);云南省科技专项(2008QA008);云南省教育厅基金(2011Y442)

摘　　要：通过扫描电子显微镜观察花粉粒的形态特征,采用I2-KI染色法测定云南黄连花粉活力,并通过联苯胺+过氧化氢反应液和3%的过氧化氢测定其柱头可授性。结果表明:云南黄连的花粉粒为近圆球形,具有萌发孔,表面具有刺状雕纹;花粉活力一天中最强的时间段是13∶30—15∶30,最强的时期是散粉时期;I型(雌蕊长于雄蕊)的云南黄连柱头可授性高于II型(雄蕊长于雌蕊)的。在散粉初期,云南黄连普遍具有较高的柱头可授性。The pollen submicroscopic morphology of Coptis teeta Wall was observed with scanning e- lectron microscopy （SEM） . The pollen vitality of C. teeta was evaluated by 12 -KI. Stigma recep- tivity was estimated by the method of Benzidine-hydrogen peroxide and 3% hydrogen peroxide. The results showed that the shape of pollen grain of C. teeta was nearly spherical and the appearance of pollen grain has germination pore with spiny. The 13： 30--15：30 was the time of the strongest pol- len vitality in a day by using 12 - KI for C. teeta. The period for the strongest pollen vitality was pol- linating period, the pollen receptivity of type I （pistil longer than stamen） was stronger than that of type Ⅱ （ stamen longer than pistil）. The pollen receptivity of C. tecta generally was higher in early pollinating period.

关 键 词：云南黄连 花粉粒 花粉活力 柱头可授性 

分 类 号：S567.520.24[农业科学—中草药栽培]