高效液相色谱法同时测定食品中的多种着色剂  

Simultaneous Determination of Various Colorants in Food by High Performance Liquid Chromatography

作　　者：张常虎[1] 翟云会[1] 

机构地区：[1]西安文理学院化学与化学工程学院,陕西西安710065

出　　处：《化学与生物工程》2012年第11期87-90,共4页Chemistry & Bioengineering

基　　金：2011陕西省教育厅科技计划项目(11JK0574);陕西省分析化学重点学科基金资助项目

摘　　要：建立了一种高效液相色谱法同时测定冰淇淋、乳酸菌饮品中的柠檬黄、苋菜红、胭脂红、日落黄、诱惑红等5种着色剂的方法。确定的色谱条件为:选用Shim-pack VP-ODS色谱柱、紫外检测器,检测波长210nm,以pH=6的甲醇-0.02mol.L-1乙酸铵溶液(20∶80,体积比)为流动相,采用等浓度洗脱,流速1.0mL.min-1。检测的线性范围为0～50μg.mL-1,相关系数为0.91185～0.9979,最低检测限为2～5μg.mL-1,回收率在95.62%～100%之间。该方法精密度高、操作简单、检测快速,是同时检测食品中多种着色剂的有效方法。A HPLC method was developed for simultaneous determination of five colorants in ice cream and lactobacillus drink such as lemon yellow, amaranth, ponceau 4R, sunset yellow and allura red. The chromato- graphic conditions were as follows ： Shim-pack VP-ODS （4.6 mm ×150 mm, 5 μm） as separation column, iso- cratic elution was adopted,methanol-0.02 mol·L-1 ammonium acetate （pH=6,volume ratio 20 ： 80）as mobile phase with the flow rate of 1.0 mL·min-1 , detection wavelength of 210 nm by UV detector. Under above con- ditions, the linear range was 0-50 μg ·mL-1 , the linear correlation coefficient was 0. 91185-0. 9979, the detec- tion limit was 2-5 μg·mL-1 ,and the recovery was 95.62%-100%. This method has high precision with the characters of simple operation and rapid determination. It is an effective method for determining various color- ants in food simultaneously.

关 键 词：高效液相色谱 同时测定 紫外检测器 精密度 

分 类 号：O657.72[理学—分析化学]