七氟醚对肢体缺血再灌注大鼠肝损伤的影响  

Effects of sevoflurane on the liver injury following ischemia-reperfusion of hind limbs in rats

作　　者：周少朋[1] 杨禄坤[1] 肖笑雨[1] 刘璐[1] 刘华[1] 

机构地区：[1]中山大学附属第五医院麻醉科,珠海市519000

出　　处：《中华普通外科学文献（电子版）》2012年第6期18-20,44,共4页Chinese Archives of General Surgery(Electronic Edition)

摘　　要：目的探讨七氟醚对肢体缺血再灌注大鼠肝损伤的影响。方法健康SD大鼠24只,体重200～250g,随机分为3组,各8只:假手术组(Sham组),只进行手术操作不做其他处理;肢体缺血再灌注组(IR组),双后肢缺血4h、再灌注6h;七氟醚组(S组)于再灌注前吸入2.5%七氟醚6h。IR、Sham组只吸入氧气。实验结束断头处死大鼠,于下腔静脉取血测定血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)的含量。摘取肝脏测定肝组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)的含量,光学显微镜下观察组织病理学结果。结果与Sham组比较,IR组和S组ALT、AST、MDA、TNF-α和IL-6含量升高,SOD含量降低(P<0.01),肝组织损伤明显;与IR组比较,S组ALT、AST、MDA、TNF-α和IL-6含量降低,SOD含量升高(P<0.05),肝组织损伤减轻。结论七氟醚对肢体缺血再灌注大鼠肝损伤具有一定程度的保护作用,其机制可能与其减少氧自由基的释放和抑制炎症反应有关。Objective To investigate the effects of sevoflurane on liver injury induced by ischemia- reperfusion of hind limbs. Methods Twenty-four healthy male SD rats weighing 200-250 g were randomly assigned into 3 groups （n = 8 each） ： sham operation group （group Sham）, ischemia-reperfusion group （group IR） and sevoflurane group （group S）. In group Sham, the bilateral femoral artery and vein were exposed but not occluded. In group IR, the bilateral femoral artery and vein were occluded for 4 h with the atraumatic microclips and then released for 6 h reperfusion. In group S, sevoflurane was inhaled for 6 h at the end-tidal concentration of 2.5% before reperfusion. In group Sham and IR the rats were inhaled oxygen instead of sevoflurane. All rats were sacrificed at 6 h of reperfusion and the blood samples were collected from the inferior vena cava to measure the concentration of serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST）. The liver tissues were obtained for determination of MDA content, SOD activity, TNF-α content, IL-6 content and microscopic examination. Results Compared with group sham, the content of ALT, AST, TNF-α, IL-6 and MDA significantly increased, SOD content significantly decreased （P 〈 0.01 ）, and pathological injury was severer in group IR and group S. Compared with group IR, the content of ALT, AST, TNF-α, IL-6 and MDA significantly decreased, SOD content significantly increased （P 〈 0.05）, and pathological injury was milder in group S. Conclusion Sevoflurane can reduce the liver injury induced by ischemia-reperfusion of hind limbs, and the mechanism may be connected with the reduce oxygen radicals and the inhibition of inflammatory reaction.

关 键 词：七氟醚 后肢 再灌注损伤 肝脏 

分 类 号：R965[医药卫生—药理学]