IL-23在鼻息肉中的表达及其意义  

Expression of IL-23 in nasal polyps and its significance

作　　者：丁国臣[1] 王亚辉[1] 司迎[2] 王艳[1] 曹志伟[3] 

机构地区：[1]武警辽宁总队医院耳鼻喉科,沈阳110034 [2]武警辽宁总队卫生处,沈阳110034 [3]中国医科大学附属盛京医院耳鼻咽喉科,沈阳110004

出　　处：《武警医学》2012年第11期976-978,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的检测IL-23在鼻息肉(nasal polyp,NP)组织中的表达,探讨其在鼻息肉发病中的作用。方法收集15例鼻息肉标本(NP组)和15例在鼻中隔偏曲矫正手术中根据需要部分切除下鼻甲黏膜组织(对照组),分别采用免疫组织化学方法和RT-PCR方法检测两组IL-23蛋白和IL-23p19mRNA表达水平。结果 NP组IL-23蛋白阳性表达炎性浸润细胞数明显多于对照组,单位目标面积光密度均值(0.321±0.023)明显高于对照组(0.199±0.011),差异有统计学意义(P<0.01);IL-23p19mRNA表达水平(0.676±0.077)明显高于对照组(0.212±0.015),差异有统计学意义(P<0.01)。结论IL-23可能在参与了NP发病过程。Objective To investigate the expression and role of interleukin-23（IL-23） in nasal polyp patients.Methods NP tissues of 15 nasal polyp（NP） patients and 15 simple deviations of normal inferior turbinate tissues of nasal septum patients were collected.mRNA and protein expression of IL-23 were detected by means of immunohistochemistry and RT-PCR.Results Immunohistochemistry staining demonstrated that there were far more IL-23 positive cells in NP patients than in normal controls.The average expression level of IL-23 protein in NP tissues（0.321±0.023） was significantly different（P0.01） from that of normal inferior turbinate tissues（0.199±0.011）.RT-PCR demonstrated that the average expression level of IL-23p19mRNA in NP tissues（0.676±0.077） was significantly different（P0.01） from that in normal inferior turbinate tissues（0.212±0.015）.Conclusions IL-23 may be involved in the chronic inflammation of NP.

关 键 词：白细胞介素23 鼻息肉 免疫组化 逆转录聚合酶链反应 

分 类 号：R765.22[医药卫生—耳鼻咽喉科]