高温处理诱导染色体加倍获得白杨杂种多倍体  

POLYPLOID INDUCTION OF Populus HYBRID THROUGH ZYGOTIC CHROMOSOME DOUBLING WITH HIGH-TEMPERATURE TREATMENT

作　　者：石乐[1,2,3] 张平冬[1,2,3] 毛彦科[1,2,3] 董春波[1,2,3] 索玉静[1,2,3] 康向阳[1,2,3] 

机构地区：[1]北京林业大学林木育种国家工程实验室,北京100083 [2]北京林业大学林木、花卉遗传育种教育部重点开放实验室,北京100083 [3]北京林业大学生物科学与技术学院,北京100083

出　　处：《核农学报》2012年第8期1118-1123,共6页Journal of Nuclear Agricultural Sciences

基　　金：林业公益性行业科研专项(20100400900-3);国家自然科学基金(31000306);中央高校基金科研业务费专项基本资助(HJ2010-15)

摘　　要：在掌握毛新杨×新疆杨合子有丝分裂规律基础上,对高温处理诱导毛新杨×新疆杨合子染色体加倍的技术条件进行研究。结果表明,在温室水培条件下,毛新杨×新疆杨合子第一次有丝分裂主要在授粉后7d;施加高温处理可有效诱导毛新杨×新疆杨合子染色体加倍,最高四倍体诱导率为7.4%,处理温度以39℃为宜;由于胚珠发育过程具有极强的不同步性,在诱导合子染色体加倍的同时还获得了部分三倍体植株。子代气孔特征观察显示:四倍体和三倍体植株都表现出多倍体特征,随着倍性水平增加,气孔增大,单位面积气孔数减少,与二倍体有显著差异。Based on zygote development,pollinated catkins of（Populus tomentosa × P.bolleana） ×P.bolleana were treated with high temperature to induce zygote chromosome doubling.In the greenhouse,the first mitotic division of zygote of（P.tomentosa × P.bolleana） ×P.bolleana occurred mainly 7d after pollination.39℃ was suitable for zygote chromosome doubling of（P.tomentosa × P.bolleana） × P.bolleana,with the best rate of tetraploid production of 7.4%.However,some triploids were formed owing to highly asynchronous development of ovule.ANOVA analysis showed that both stomata size and density were significant difference among diploid,triploid and tetraploid plants.

关 键 词：毛新杨 多倍体诱导 高温 气孔特征 

分 类 号：S792.11[农业科学—林木遗传育种]