山茱萸籽油的响应曲面优化微波辅助提取及成分分析  

Microwave-assisted extraction optimized with response surface methodology and component analysis of Cornus officinalis seed oil

作　　者：刘瑞林[1] 詹汉英[1] 陈钿[1] 牟朝丽[1] 朱苏骐[1] 张志琪[1] 

机构地区：[1]药用资源与天然药物化学教育部重点实验室,陕西师范大学化学化工学院,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2012年第6期47-51,60,共6页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(20875060)

摘　　要：研究以正己烷为溶剂微波辅助提取山茱萸籽油的工艺并对其化学成分进行分析.采用响应曲面优化法(RSM)优化山茱萸籽油的提取工艺,并利用GC-MS、分光光度法对山茱萸籽油中脂肪酸成分及总多酚含量进行分析.建立了一个较好预测试验结果的模型方程,根据该方程对山茱萸籽油的微波辅助提取工艺参数进行优选,确定了其最佳工艺为:微波功率640 W,提取时间13min,液料比12∶1(mL/g),在该条件下的一次提取率为10.18%.GC-MS分析鉴定出14种化学成分,主要脂肪酸成分为油酸(61.62%)、硬脂酸(21.99%)、棕榈酸(7.54%)和亚油酸(4.31%)等,其中不饱和脂肪酸占脂肪酸总量的67.05%;山茱萸籽油中总多酚类化合物含量以没食子酸表示为0.42mg/g.To study the microwave-assisted extraction technology and component analysis of Comus officinalis seed oil, the best extraction parameters were obtained with response surface methodology （RSM）, and the fatty acid composition and polyphenol content were analyzed with GC-MS and spectrophotometry. Meanwhile,a better predicted model equation was established for the results of the experiment. According to this equation, the microwave-assisted extraction parameters were optimized, and the optimal technology parameters as follows were obtained： microwave power, 640 W; extraction time, 13 mini and solvent to sample ratio, 12 ＂ 1 （mL/g）. Under these conditions, the extraction yield of Comus officinalis seed oil was 10.18%. In total, 14 kinds of chemical components were identified, the main fatty acids in the oil are oleic （61.62%）, stearic acid （21.99%）, palmitic acid （7.54%）, linoleic acids （4.31%） and so on. The content of unsaturated fatty acids in total fatty acids is 67.05%. In addition, the content of polyphenon as gallic acid in the oil is 0.42 mg/g.

关 键 词：山茱萸籽油 微波辅助提取 响应曲面法 成分分析 

分 类 号：O657.3[理学—分析化学]