人结直肠癌细胞Annexin A2基因敲除细胞系的建立  

The generation of Annexin A2-/-Caco2 cell line

作　　者：邢睿欢[1] 牟茂森[1] 高晓杰[1] 聂红娟[1] 杨敏敏[1] 徐竹[1] 熊艳[1] 吴琴丰[1] 陈亦雯[1] 宋铭[1] 侯颖春[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2012年第6期61-66,共6页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：陕西省自然科学基金资助项目(SJ08-ZT09)

摘　　要：为研究ANXA2与结直肠癌发展的关系以及其在肿瘤发展过程中作用的构效关系,构建了ANXA2基因敲除质粒重组子,然后导入人结直肠癌细胞系Caco2,经过一系列筛选过程,最终建立了基因敲除ANXA2的Caco2细胞系(ANXA2-/-Caco2).经过PCR和Western Blotting检测,确认该细胞系无任何野生ANXA2表达.To study the roles played by ANXA2 during the development of colorectal carcinoma, and the relationship between the structure and function of ANXA2, ANXA2 knockout recombinant was constructed and transfected into Caco2 cell line. After a series of selecting step, ANXA2 --/--Caco2 cell line was successfully generated. By the designated PCR and Western blot analysis, it was confirmed that the wild type ANXA2 expression is knockout completely in this cell line.

关 键 词：AnnexinA2 基因敲除 Caco2细胞 

分 类 号：Q291[生物学—细胞生物学]