秦艽HMGR基因家族的克隆及序列分析  

Cloning and characterization of HMGR genes from Gentiana macrophylla

作　　者：郑鹏[1,2,3] 化文平[1,2,3,4] 王喆之[1,2,3] 

机构地区：[1]教育部药用资源与天然药物化学重点实验室 [2]西北濒危药材资源开发国家工程实验室 [3]陕西师范大学生命科学学院,陕西西安710062 [4]陕西学前师范学院生物科学与技术系,陕西西安710100

出　　处：《陕西师范大学学报（自然科学版）》2012年第6期67-72,共6页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(31000134);陕西省教育厅科研项目(12JK0833);陕西学前师范学院科研项目(2012KJ044)

摘　　要：研究克隆了秦艽3-羟基-3-甲基戊二酰辅酶A还原酶(HMGR)家族成员的两条基因,并对其序列进行了生物信息学分析.结果显示,两条HMGR基因都包含完整的开放读码(ORF)框,编码蛋白含有HMGR蛋白特有的4个保守的活性位点,与其他植物HMGR蛋白序列具有较高的一致性,都具有跨膜结构域,高级结构类同;GmHMGR1和GmHMGR2分别定位于细胞质膜和线粒体中.表达分析显示,GmHMGR1主要在根和花中表达,而GmHMGR2主要在叶中表达.Gentiopicroside, a secoiridoid glycoside, is one of the main pharmacological compounds of traditional Chinese medicine Gentiana. In this study, two Gentiana macrophylla HMGR genes were cloned based on our laboratory＇s high-throughput sequencing, and bioinformatics analysis was carried out. The results showed that two HMGR genes both contain the completed ORF, and their encoded proteins with two transmembrane domains and four HMGR protein-specific conserved active sites, high consistency with the other plant in HMGR protein sequences and high structure, but GrnHMGR1 and GmHMGR2 locate respectively in the cell plasma membrane and mitochondria, Quantitative PCR analysis showed that GmHMGRI has high expression levels in the roots and flowers in G. macrophylla, while GmHMGR2 mainly expresses in leaves.

关 键 词：秦艽 3-羟基-3-甲基戊二酰辅酶A还原酶(HMGR) 高通量测序 序列分析 

分 类 号：Q274[生物学—细胞生物学]