重组人IFN-γ对抗原SC3A表达的上调作用  

Up-regulation of antigen SC3A-expressing with rhIFN-γ

作　　者：姜志明[1] 刘民培[1] 曹建平[1] 张晓晨[1] 孟露露 金伯泉[1] 刘亚革[1] 麻树人[1] 丁辽平[1] 赵黎明[1] 陈晓琳[1] 

机构地区：[1]解放军沈阳军区总医院,辽宁沈阳110015

出　　处：《现代康复》2000年第6期862-863,共2页Modern Rehabilitation

基　　金：辽宁省自然科学基金资助项目!(962251)

摘　　要：目的 :探讨重组人IFN -γ(rhIFN -γ)在体外对SW1116抗原SC3A表达的影响。方法 :将rhIFN -γ与SW1116在37℃共育24、48和72h后 ,分别用免疫细胞化学染色法和流式细胞仪测定其SC3A抗原 (SC3AAg)的表达。结果:SW1116与20u/mlrhIFN -γ共育24、48和72h ,其SC3AAg 阳性细胞分别为95.5 %、93.2 %和95.2 %,PBS组分别为11.3 %、12.2 %和10.0 % ,AGZY -83 -a细胞系均未见SC3AAg 阳性细胞 ,阳性对照组的C1184细胞系SC3AAg阳性细胞80.5 %。结论 :2u/mlrhIFN -γ能使SW1116细胞的SC3AAg表达明显上调 ,其中20u/ml、24h是表达峰值的最佳浓度和时间。Objective:To observe regulation of the tumor associated antigen SC3A(TAASC3A)on cell line SW1116,human colon carcinoma,using rhIFN-γin vitro.Method:The cell line SW1116 were induced with rhIFN-γ.The percentage of positive cells with antigen SC3A(SC3AAg)expressing was determined by immunohistochemistry staining and Flow cytometry(FCM)respectively.Results:The positive cells of SC3AAgexpressing on/in SW1116 induced by 20u/ml rhIFN-γwere 95.5%,93.2%and 95.2%respectively at 24,48 and 72 h.SC3AAg positive cells of SW1116 without rhIFN-γwere 11.3%,12.2%and 10.0%respectively.No SC3AAgexpressing on AGZY83a was observed.Conclusion:2u/ml of rhIFNγcan regulate up SC3AAg on SW1116 obviously.It was showed that 20 u/ml of rhIFN-γfor 24h was optimum concentration and time.

关 键 词：重组人INF-γ 抗原SC3A SW1116细胞 

分 类 号：R392.1[医药卫生—免疫学]