四川黑熊RPS16亚基基因的克隆与序列分析  

CDNA Cloning and Sequence Analysis of Ribosomal Protein S16 Gene from Sichuan Black Bear(Ursus thibetanus mupinensis)

作　　者：王婷[1] 侯怡铃[1] 丁祥[1] 孙冰[1] 苏秀兰[1] 李俊[1] 侯万儒[1] 

机构地区：[1]西华师范大学生命科学院,四川南充637009

出　　处：《西华师范大学学报（自然科学版）》2012年第4期350-355,共6页Journal of China West Normal University(Natural Sciences)

基　　金：四川省应用技术资助项目(2011JY0135);四川省教育厅重点科研项目(07ZA120);四川省重点学科动物学建设经费资助(404001);四川省应用基础项目(2009JY0061);四川省教育厅青年基因项目(092B088);四川省教育厅项目(10ZC120)

摘　　要：根据已报道的部分哺乳动物RPS16基因的相关信息设计引物,运用RT-PCR技术,从四川黑熊的肌肉组织总RNA中对核糖体蛋白亚基RPS16基因的表达序列进行克隆、测序和分析.结果表明:四川黑熊RPS16基因的表达序列全长为481bp,开放阅读框(ORF)为441bp,编码146个氨基酸,相对分子质量为16.4KDa,pI为10.81.拓扑预测显示含有6个类型的功能位点:1个依赖cAMP和cGMP的蛋白激酶磷酸化位点,3个蛋白激酶C磷酸化位点,1个酪蛋白激酶II磷酸化位点,2个N-糖基化位点,1个乙酰化位点及1个核糖体蛋白S9信号位点.进一步分析发现,四川黑熊RPS16基因与已报道的大熊猫、牛、野猪、人、小家鼠和褐家鼠等6个哺乳动物物种的表达序列及其编码的氨基酸序列有很高的相似性,说明RPS16基因具有高度的遗传稳定性和功能一致性.The cDNA of RPS16 was cloned from Sichuan black bear by RT-PCR. The result shows that the full length of the eDNA fragment cloned is 481bp in size containing an open reading frame of 441bp. The encoded protein is composed of 146 amino acids and its estimated molecular weight is 16. 4424kD with a pI of 10.81. Topology prediction shows that there are 6 different patterns of functional sites： one cAMP-and eGMP-dependent protein kinase phosphorylation site ; three Protein kinase C phosphorylation site;one Casein kinase II phosphoryiation site ;two N-myristoylation site ;one Amidation site and one Ribosomal protein S9 signature. Further analysis shows that the sequences and encoded amino acids in RPS16 have a high homology with Ailuropoda melanoleuca, Bos Taurus, Sus scrofa ,Homo sapiens, Mus musculus and Rattus norvegicus which indicates that the genetic stability and function of RPS16 gene in Sichuan black bear are consistent with those of RPS16 gene reported in mammals.

关 键 词：四川黑熊(Ursus thibetanus mupinensis) RT—PCR RPS16 克隆 序列分析 

分 类 号：Q778[生物学—分子生物学]