水稻磷高效基因的初步定位  

Molecular Mapping of Phosphorus-Efficient Gene in Rice

作　　者：易俊良[1,2] 周德贵[2] 卢德城[2] 吴耀荣[3] 李宏[2] 黄道强[2] 赖穗春[2] 王重荣[2] 王志东[2] 陈立云[1] 谢旗[3] 周少川[1,2] 

机构地区：[1]湖南农业大学水稻科学研究所,长沙410128 [2]广东省农业科学院水稻科学研究所,广州510640 [3]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《分子植物育种》2012年第6期628-634,共7页Molecular Plant Breeding

基　　金：比尔和梅琳达.盖茨基金会项目(51587-13);农业部948项目(2006-G1)共同资助

摘　　要：磷是植物生长发育所必需的大量营养元素。植物磷营养高效利用通常与根的形态、根分泌物、膜与体内磷转运以及菌根等因素有关,表现为受多基因控制。本文通过前期筛选工作,获得1份耐低磷株系。利用230对SSR和InDel引物,对低磷材料所在的回交导入系群体进行了初步定位,定位结果显示,与磷利用效率相关的基因座位有两个OsPe5和OsPe7,分别位于第5染色体的InDel520与InDel529标记之间,与InDel525标记共分离,物理距离约为900kb,和第7染色体InDel703与InDel717标记之间,与InDel713标记共分离,物理距离约为1400kb。Phosphorus（P） was an essential macronutrient for plant growth and development.High-efficient utilization of P-nutrition in plant was closely related to many factors,such as root morphology,root exudates,P-transportors across plasmalemma,P-translocation and symbiosis with microorganisms and so on.These nutritional properties were genetically controlled by multi-genes and characterized by quantitative trait loci（QTLs）.In this study,a line with tolerance to low P was selected based on the identification of previous work.Backcross introgression lines（BIL） population with low-phosphorus materials were used for preliminary mapping by using 230 pairs of SSR and InDel primers.The results showed that two loci were associated with P utilization efficiency and mapped between the markers of InDel520 and InDel529 on the chromosome 5 and InDel703 and InDel717 on the chromosome 7,respectively.The locus on the chromosome 5 was co-segregated with the InDel525,about 900 kb physical distance,wher eas the locus on the chromosome 7 was co-segregated with the InDel713,approximately 1 400 kb physical distance.

关 键 词：水稻 磷高效利用 近等基因导入系 基因定位 

分 类 号：S511[农业科学—作物学]