与甜瓜嫁接亲和性连锁分子标记鉴定  

Identification of Molecular Marker Linked to Grafting Compatibility in Melon

作　　者：陈亚丽[1,2] 郭世荣[1] 朱为民[2] 刘龙洲[2] 陈幼源[2] 

机构地区：[1]南京农业大学园艺学院,农业部南方蔬菜遗传改良重点开放实验室,南京210095 [2]上海市农业科学院设施园艺研究所,上海市设施园艺技术重点实验室,上海201403

出　　处：《分子植物育种》2012年第6期714-721,共8页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31000915);上海市重点学科(蔬菜学-交通大学)建设项目(B209);上海市启明星人才计划(11QA1405700)共同资助

摘　　要：使用与南瓜类型砧木"(壮士")嫁接亲和性差异明显的两个甜瓜自交系(85-1和B717)为亲本,配制F2:3家系群体;利用BSA法构建亲和性优差池,结合PCR技术筛选与嫁接亲和性有关的分子标记。结果表明:使用葫芦科遗传连锁图谱上发表的750对SSR、SRAP和AFLP等分子标记引物扩增,最终筛选出在两个甜瓜材料之间具有多态性的382对引物,其中9对引物分别在嫁接亲和性优差DNA池中扩增出差异性片段。将这些标记用于F2群体的扩增,计算标记与嫁接亲和性遗传连锁距离,两对SSR引物CMN-0616和M103与嫁接亲和性的遗传距离分别为17.6cm和9.3cm。这2对引物有望成为鉴定甜瓜嫁接亲和性的辅助引物,从而将嫁接亲和性差异材料从分子角度区别开来,为甜瓜嫁接接穗育种等研究提供参考。Two melon varieties（85-1 and B717）,being significant differences in grafting affinitywith the pumpkin rootstock ＂a strong man＂,were used as parents to construct F2：3 populations;molecular markers linked to graft compatibility were identified by PCR technology based on differential DNA pools in grafting affinity prepared by BSA approach.The results showed that 382 pairs of primers with polymorphism in two melon parents were screened from 750 pairs of SSR,SRAP,AFLP primers,which had published in the cucurbitaceae genetic linkage map.There are 9 pairs of primers that can respectively amplified different fragments in differential DNA pools in grafting affinity,and these different markers were further used to amplify F2 population to calculate the genetic linkage distance between the marker and the trait of grafting affinity.The distance of markers of CMN-0616 and M103 both linked to grafting affinity with the genetic were 17.6cm and 9.3cm respectively.These two pairs of primers would be expected to be as assist markers to identify grafting affinity of the melon in order to distinguish varieties with different grafting affinity at the level of molecular background which might provide the reference for developing grafted melon scion in breeding program.

关 键 词：甜瓜 嫁接 亲和性 分子标记 

分 类 号：S652[农业科学—果树学]