黄麻幼苗cDNA文库的构建和质量鉴定  

Construction and Evaluation of cDNA Library from Jute Seedlings

作　　者：谢小芳[1] 陈燕萍[1] 吴为人[1] 

机构地区：[1]福建农林大学生命科学学院,福州350002

出　　处：《分子植物育种》2012年第6期747-750,共4页Molecular Plant Breeding

基　　金：福建省自然科学基金项目(2012J01076)资助

摘　　要：黄麻全长cDNA文库的构建对开展黄麻遗传和功能研究具有重要的应用价值。以生产上的优良品种闽引一号为材料,利用Creator SMART cDNA ConstructionKit技术,成功构建了黄麻苗期的全长cDNA文库。文库的滴度为1.9×106pfu/mL。随机挑取86个克隆进行菌落PCR鉴定,结果显示:插入片段的大小在0.5～2.0kb之间。研究结果表明,黄麻全长cDNA文库的质量较高。Construction of the full-length cDNA library of jute is significant value in genetic and function research.With a well known jute（Corchorus capsularis L.） cultivar Minyin no.1 as material,a full-length cDNA library was constructed using Creator SMART cDNA Construction Kit technology.This library had a capacity of 1.9×106 pfu/mL and the insert sizes was about 0.5～2.0 kb based on PCR analysis of 86 randomly chosen clones.The results suggest that the cDNA library of jute should have been constructed successfully.

关 键 词：黄麻 SMART技术 CDNA文库 

分 类 号：S563.4[农业科学—作物学]