不同草地早熟禾品种愈伤组织再生体系研究  

Callus Regeneration Study of Poa pratensis L.Varieties

作　　者：雷炳琪[1] 代亮[1] 柳玉霞[1] 刘洁[1] 徐筱[1] 徐倩[1] 徐吉臣[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室,北京100083

出　　处：《分子植物育种》2012年第6期751-755,共5页Molecular Plant Breeding

基　　金：国家自然科学基金项目(J1103516;31128016);北京市自然科学基金项目(5112015)共同资助

摘　　要：草地早熟禾(Poa pratensisL.)是一种复杂的多倍体植物且具有兼性无融合生殖方式,建立高效的愈伤组织培养体系是进行基因工程育种的重要环节。本研究利用生产中常用的6个草地早熟禾品种为材料,经愈伤组织诱导后,对其愈伤组织分化及再生体系进行了研究。结果发现,不同草地早熟禾品种的分化再生能力具有明显的差异,不同激素处理组合对草地早熟禾愈伤组织的分化有显著的影响:品种如"帝王"、"超级伊克利"、"蓝鸟"等较易分化和再生,品种如"盛宴"、"布鲁克"等则只在特定条件下分化和再生;培养基激素处理组合Ⅴ(2mg/L6-BA+0.1mg/L2,4-D)较适合于所有检测的品种,处理组合Ⅳ(2mg/L6-BA)和Ⅵ(2mg/L6-BA+0.2mg/L2,4-D)对部分品种的分化有较大的促进作用。研究同时评估了2种激素配比(6-BA和2,4-D)对早地早熟禾愈伤分化再生的影响,发现不同草坪草品种对单独使用的6-BA浓度敏感性不同,补加2,4-D后不同品种的分化率也存在明显差异。研究建立的不同草地早熟禾品种的高效再生体系,不仅有利于这些品种的基因工程改造,也为草地早熟禾愈伤组织再生体系研究奠定了重要的理论基础。Poa pratensis L.is a kind of polyploid Kentucky Bluegrass plant with facultative apomictic mode of reproduction.An efficient system of callus differentiation and plant regeneration is important in the gene engineering breeding.The research here was to optimize the system in 6 Kentucky Bluegrass varieties.The results revealed that the Kentucky Bluegrass varieties had the different ability in callus differentiation and plant regeneration,and had the different sensitivity to the phytohormone treatment.The varieity of Diva,Total Eclipse and Bluebird were easier to differentiate and regenerate while Feast brand and Brook lawn were conditionally.The treatment Ⅴ（2 mg/L 6-BA＋0.1 mg/L 2,4-D） were siutful for each variety,and the treatment Ⅵ（2 mg/L 6-BA） and Ⅵ（2 mg/L 6-BA＋0.2 mg/L 2,4-D） were in more contribution to some varieties.Meanwhile,the ratio of phytohormone 6-BA and 2,4-D in medium can affect the callus differentiation significantly.The only use of 6-BA or the further addition of 2,4-D promoted the callus differentiation differently among varieites.The efficient differentiation system established for each Kentucky Bluegrass variety here would be useful for their gene engeering breeding program,and also be an important theoretical basis for the differentiation theory study in Kentucky Bluegrass.

关 键 词：草地早熟禾 组织培养 再生 品种 

分 类 号：S688.4[农业科学—观赏园艺]