血管紧张素转化酶2基因过表达改善人脐静脉内皮细胞生长  

The Effects of Angiotensin-converting Enzyme 2 Gene Overexpression on the Proliferation and Apoptosis of Human Umbilical Vein Endothelial Cells

作　　者：晋学庆[1] 王前胜[1] 卢卓强[1] 王华军[1] 翁智远[1] 

机构地区：[1]福建医科大学附属第一医院,福建省高血压研究所,福州350005

出　　处：《福建医科大学学报》2012年第5期310-314,共5页Journal of Fujian Medical University

摘　　要：目的探索在血管紧张素转化酶2(ACE2)基因过表达干预下血管紧张素(AngⅡ)对人血管内皮细胞增殖、凋亡的影响。方法原代培养人脐静脉血管内皮细胞。慢病毒重组ACE2基因表达载体(Lentiviral-ACE2)以感染复数为10(MOI=10)感染人脐静脉内皮细胞(HUVECs)。感染72h后,以AngⅡ(终浓度为10-7mol/L)干预细胞,倒置相差显微镜观察HUVECs的形态学变化,AlamarBlue试剂检测HUVECs增殖功能,TUNEL法检测HUVECs凋亡。结果 AngⅡ组与AngⅡ+Lentiviral-GFP组细胞生长状态差,生长缓慢,形态不规则并且有不同程度脱壁悬浮的细胞;而正常细胞组与AngⅡ+Lentiviral-ACE2组细胞生长状态良好。AngⅡ组较正常细胞组、AngⅡ+Lentiviral-ACE2组AlamarBlue被还原率明显降低。AngⅡ组的凋亡指数(0.11±0.02)与正常细胞组(0.04±0.01)和AngⅡ+Lentiviral-ACE2(0.05±0.01)比较,具有统计学意义(P<0.05)。AngⅡ组与AngⅡ+Lentiviral-GFP比较、正常细胞组与AngⅡ+Lentiviral-ACE2组比较,AlamarBlue被还原率和凋亡指数差别无统计学意义(P>0.05)。结论 AngⅡ具有降低人脐静脉内皮细胞增殖能力和增加细胞凋亡的作用,ACE2过表达改善AngⅡ诱导的人脐静脉内皮细胞增殖和减少细胞凋亡,对人脐静脉内皮细胞具有保护作用。Objective To explore the effects of overexpression of angiotensin-convrting enzyme 2（ACE2） on the proliferation and apoptosis induced by angiotensin II in human umbilical vein endothelial cells（HUVECs）.Methods In vitro cultured HUVECs were infected with recombinant lentiviruses expressing ACE2（Lentiviral-ACE2） for 72 hours with an multiplicity of infection of 10（MOI=10）;HUVECs were then treated with AngⅡ at a final concentration of 10-7mol/L.The morphological changes of HUVECs in each group were observed by inverted phase contrast microscope;the proliferation of HUVEC in each group was detected by Alamar Blue reagents;and the apoptosis in each group was assayed by TUNEL Apoptosis Detection Kit.Results Compared to untreated normal control cells and AngⅡ＋Lentiviral-ACE2 cells,cells in AngⅡ group and AngⅡ ＋ Lentiviral-GFP group were in poor growing conditions with slow doubling time,irregular cell morphologies;lots cells were floating in the culture medium;Alamar Blue reduction rate in AngⅡ group was significantly lower（P0.05） than those of normal cell control group and AngⅡ＋Lentiviral-ACE2 group.Apoptosis index（0.12±0.02） in AngⅡgroup was also statistical significant higher（P0.05） compared to those in normal cell control group（0.04±0.01） and AngⅡ＋ Lentiviral-ACE2 group（0.05±0.01）.However,the Alamar Blue reduction rate and apoptosis index between AngⅡ group and AngⅡ ＋ Lentiviral-GFP and between normal cells control group and AngⅡ＋Lentiviral-ACE2 were not statistically significant different.Conclusion AngⅡ reduces proliferation and increases apoptosis of human umbilical vein endothelial cells,while the overexpression of ACE2 protects AngⅡ-treated HUVEC by increasing proliferation and decreasing apoptosis.

关 键 词：肽基二肽酶A 内皮 脐静脉 血管紧张素Ⅱ 增殖 凋亡 

分 类 号：R543.5[医药卫生—心血管疾病]