骨形态发生蛋白-α-氰基丙烯酸复合生物胶修复大鼠下颌骨骨缺损的研究  

Compound bone morphogenetic protein-alpha-cyanoacrylate biological glue repairs mandibular defects in rats

作　　者：王新[1,2] 谢富强[2] 张贇 孙健[2] 

机构地区：[1]兰州大学口腔医学院,甘肃省兰州市730000 [2]兰州大学第二医院口腔科,甘肃省兰州市730030

出　　处：《中国组织工程研究》2012年第43期7986-7990,共5页Chinese Journal of Tissue Engineering Research

基　　金：甘肃省科技支撑计划(1011FKCA103);甘肃省卫生行业科研计划资助项目(GSWST2011-09)~~

摘　　要：背景:骨形态发生蛋白2是骨形态蛋白家族中诱导成骨作用最强,并能单独诱导成骨的因子。目的:观察骨形态发生蛋白-α-氰基丙烯酸正丁酯复合生物胶修复大鼠下颌骨缺损的效果。方法:将54只Wistar大鼠随机分为实验组、对照组、空白对照组。3组均制作下颌骨缺损模型,实验组缺损处植入骨形态发生蛋白-α-氰基丙烯酸复合生物胶,对照组植入壳聚糖温敏性凝胶,空白对照组不植入任何材料。结果与结论:①X射线片检查:植入后3,6,9周时,实验组剩余骨缺损面积均低于对照组及空白对照组(P<0.05)。②组织学观察:病理切片显示,实验组下颌骨成骨情况优于对照组及空白对照组。表明骨形态发生蛋白-α-氰基丙烯酸复合生物胶具有良好的生物相容性和骨诱导性,可显著促进颌骨缺损修复。BACKGROUND： Bone morphogenetic protein 2 is the strongest in the family of bone morphogenetic proteins which can induce osteogenesis separately. OBJECTIVE： To evaluate the effectiveness and feasibility of implantation of the compound bone morphogenetic protein- cyanoacrylate biological glue in the reconstruction of rat mandibular defects. METHODS： Totally 54 male Wistar rats were divided into three groups randomly and equally： The experimental group, the control group （the chitosan group） and the blank group. Mandibular defects of rats were repairing by implanting the compound bone morphogenetic protein-α-cyanoacrylate biological glue in the experimental group. While mandibular defects of the chitosan group were repaired only with chitosan. The blank group received no implantation. RESULTS AND CONCLUSION：（1）X-ray examination： At weeks 3, 6, 9 postoperation, the area of residual mandibular defects in the experimental group was smaller than that of the other two groups. There was a statistically significant difference between the experimental and the other two groups （P 〈 0.05）. （2）Histopathology observation： The new bone mass in the experimental group was superior to those in the other two groups. It is demonstrated bone morphogenetic protein α-cyanoacrylate biological glue can be used to restoration of rat mandibular bone defects and significantly promote the mandibular repair.

关 键 词：骨形态发生蛋白2 α-氰基丙烯酸酯生物胶 壳聚糖 下颌骨缺损 修复 

分 类 号：R318[医药卫生—生物医学工程]