纳米银缓释壳聚糖/聚乙烯醇伤口敷料的制备及表征  

Preparation and characterization of nano-silver slow-release chitosan/polyvinyl alcohol sponge wound dressings

作　　者：许为康[1,2,3] 魏坤[1,2,3] 曾晓峰[1,2,3] 凌友[1,2,3] 张捷[1,2,3] 魏欣苗[1,2,3] 

机构地区：[1]华南理工大学材料科学与工程学院,广东省广州市510640 [2]国家人体组织功能重建工程技术研究中心,广东省广州市510006 [3]广东省生物医学工程重点实验室,广东省广州市510006

出　　处：《中国组织工程研究》2012年第43期8023-8026,共4页Chinese Journal of Tissue Engineering Research

基　　金：基金声明:国家"973"计划项目(A00102110400)资助~~

摘　　要：背景:临床上传统治疗创面感染主要使用抗生素药物敷料,但长期使用会诱导细菌耐药;同时纳米创伤敷料生物相容性差,无法降解,不宜长期覆盖创面。目的:制备一种具有抗菌作用、生物相容性良好的伤口敷料,并表征分析其生物学性能。方法:采用缩醛化反应制得含纳米银/聚乙二醇的壳聚糖/聚乙醇酸海绵,检测材料的物理性能、表面形貌、体外释放及抗菌性能。结果与结论:制得的纳米银粒径小,分散性良好,体外释放实验表明纳米银粒子可持续不断地从辅料释放出来,作用于细菌。含纳米银/聚乙二醇的壳聚糖/聚乙烯醇海绵孔隙致密均匀,大小孔相互贯通;吸水性大、保湿性高、透气性适中;吸水率和透气率都随聚乙烯醇1799含量增加而增大;保湿率基本不变;对金黄色葡萄杆菌、大肠杆菌、白色念珠菌、铜绿假单胞菌、变伤寒沙门菌5种实验菌种均有良好杀菌效果。表明该敷料物理性能好,生物相容性及杀菌效果好。BACKGROUND： The clinical treatment of traditional wound infection is mainly use antibiotic drugs dressings, but the long-term use can cause bacteria resistance. Meanwhile, nano wound dressings used in clinical application has poor biocompatibility and cannot be degraded, which should not be used for long-term coverage of wound. OBJECTIVE： To prepare a wound dressing with antibacterial action and good biocompatibility, and to characterize and analysis its biological properties. METHODS： Chitosan/polyvinyl alcohol sponge containing nano-silver/polyethyleneglycol was prepared by acetalization reaction. The material＇s mechanical properties, surface topography, in vitro release, and antibacterial properties were detected. RESULTS AND CONCLUSION： The particle diameter of nano-silver prepared was small and it had good disparity. The in vitro release studies showed that Ag nano-silver particles were able to release continuously and sterilize the bacterium. The Chitosan/polyvinyl alcohol sponge containing nano-silver/polyethyleneglycol had dense and uniform interspaces; the pores inside was interconnected, and had favorable moisture-adsorption rate, moisture-retention rate, and moderate air permeability rate. The moisture-adsorption rate and air permeability rate were increased with the content of polyvinyl alcohol 1799 increased, but the moisture-retention rate almost had no effects with the changes of the content of polyvinyl alcohol 1799. The material had a good bactericidal effect on the staphylococcus aureus, escherichia coil, candida albicans, pseudomonas aeruginosa and salmonella typhi. These findings suggest that the dressing not only possesses good physical properties and anti-microbial, but also its synthesis procedure is simple and low cost. Therefore, the developed material can be applied to new wound dressing.

关 键 词：纳米银 聚乙二醇 聚乙烯醇 壳聚糖 伤口敷料 生物材料 

分 类 号：R318[医药卫生—生物医学工程]