贵金属烤瓷合金与高糖对细胞外液炎性因子的影响  

Effects of noble ceramic alloy and high glucose on inflammatory factors in the extracellular fluid

作　　者：谢晖[1] 丁江峰[1] 

机构地区：[1]广州医学院口腔医院,广东省广州市510140

出　　处：《中国组织工程研究》2012年第43期8045-8048,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:前期研究中发现高糖与烤瓷合金浸提液对小鼠成纤维细胞L-929的增殖存在交互效应。目的:观察3种贵金属烤瓷合金与高糖对小鼠成纤维细胞L-929中白细胞介素6水平的影响。方法:采用两因素析因设计实验研究不同糖浓度(11.1,22.2,33.3mmol/L)下,89%金合金、74%金合金及银钯合金贵金属烤瓷合金浸提液,分别与小鼠成纤维细胞L-929体外共培养24h后,细胞外液中白细胞介素6水平的变化,并设未加入合金材料的空白对照为阴性对照组。结果与结论:两因素析因设计分析结果表明,贵金属烤瓷合金浸提液的主效应有统计学意义(P=0.001),其中74%金合金与银钯合金白细胞介素6水平低于阴性对照组(P=0.004,0);糖浓度的主效应也有统计学意义(P=0.005),33.3mmol/L糖浓度组白细胞介素6水平低于11.1mmol/L(正常)糖浓度组(P=0.001)。但贵金属烤瓷合金浸提液与糖浓度两因素对小鼠成纤维细胞L-929细胞外液中白细胞介素6水平的影响无交互作用(P=0.33)。BACKGROUND： The preliminary studies have found that there is an interaction effect of high sugar and ceramic alloy extracts on proliferation of L-929 mouse fibroblasts. OBJECTIVE： To observe the effects of three kinds of noble ceramic alloys and high glucose on secrets of interleukin-6 from L-929 cells. METHODS： Using two-factor factorial design experiments, we detected the changes of interleukin-6 levels in the extracellular fluid after co-culture of 89% Au-alloy, 74% Au-alloy and Ag-Pd alloy with mouse L-929 cells under different glucose concentrations （11.1, 22.2, 33.3 mmol/L）. Cells without alloy served as negative controls. RESULTS AND CONCLUSION： Two-factor factorial design results showed that the main effect of noble ceramic alloy extracts was statistically significant （P=0.001）. The interleukin-6 levels of the supernatant in the 74% Au-alloy and the Ag-Pd alloy were significantly lower than that in the negative group （P=0.004, 0）. The main effect of glucose concentration was also of statistical significance. The interleukin-6 level in supernatant in 33.3 mmol/L high glucose was significantly lower than that in 11.1 mmol/L glucose. However, no interaction existed between different extracts of noble ceramic alloys and glucose concentrations about the secrets of interleukin-6 from L-929 cells （P=0.33）.

关 键 词：贵金属 烤瓷合金 银钯合金 金合金 浸提液 细胞外液炎性因子 高糖浓度 交互作用 小鼠成纤维细胞 

分 类 号：R318[医药卫生—生物医学工程]