心肌细胞与胶原材料复合再造组织工程心肌移植心肌梗死大鼠的心肌电生理变化  

Electrophysiological changes following transplantation of tissue engineered myocardial tissue constructed by the compound of myocardial cells and collagen materials in rats with myocardial infarction

作　　者：孙娟[1] 陈晓洋[2] 张玲[1] 黄焰[3] 

机构地区：[1]新疆医科大学第一附属医院,新疆维吾尔自治区乌鲁木齐市830054 [2]新疆维吾尔自治区人民医院,新疆维吾尔自治区乌鲁木齐市830001 [3]上海华东医院,上海市200040

出　　处：《中国组织工程研究》2012年第43期8078-8082,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30960105)~~

摘　　要：背景:组织工程化心肌组织移植可修复大鼠心肌梗死。目的:应用微电极阵标测技术分析心肌细胞与胶原材料复合组织工程再造心肌移植心肌梗死大鼠的心肌电生理特性。方法:将成年SD大鼠随机分组:假手术组、模型组、移植组。后2组制作心肌梗死动物模型,假手术组仅开胸不结扎冠状动脉,移植组造模后移植心肌细胞与胶原材料复合组织。应用微电极阵标测技术记录心室肌场电位振幅,激活-恢复时间及激动传导时间。结果与结论:①心室肌场主波振幅:与模型组相比,移植组梗死区、对立区及周围区部位增高(P<0.05)。②激活-恢复时间:模型组与移植组梗死区与梗死周边区均明显延长,且梗死周边区小于梗死区(P<0.05)。与模型组相比,移植组梗死区、对立区及周围区部位激活-恢复时间缩短(P<0.05)。③心室前壁激动传导时间:移植组高于假手术组,但低于模型组(P<0.05)。表明移植的心肌细胞/胶原复合体能改善心肌梗死大鼠心室肌收缩功能及电传导时间,部分修复心脏功能。BACKGROUND： Transplantation of tissue engineered myocardial tissue can repair myocardial infarction in rats. OBJECTIVE： To study the electrophysiological characteristics after the transplantation of tissue engineered myocardia tissue. METHODS： Thirty SD rats were evenly divided into three groups： control group, model group and transplantation group. A rat model of myocardial infarction was established in the latter two groups. The control group underwent thoracotomy with no ligation of the coronary artery. The transplantation group was transplanted with the compound of myocardial cells and collagen materials after modeled. Amplitude, activation-recovery interval and activation-conduction duration at local myocardium were recorded with microeletrode arrays technique. RESULTS AND CONCLUSION： （1）Compared with the model group, the stationary field amplitude of ventricular myocardium in infarction zone, opposite zone and border zone in the transplantation group were significantly increased （P 〈 0.05）. （2）Activation-recovery interval in the model group, as well as infarction zone and infarcted border zone in the transplantation group were prolonged variously （P 〈 0.05）. While the duration of infarction zone, opposite zone and border zone in the transplantation group were shorten compared with the model group. （3）In terms of the activation-conduction duration of left ventricular anterior wall, the transplantation group was higher than the control group, but lower than the model group （P 〈 0.05）. These findings suggest that the compound of myocardial cells and collagen materials transplanted can improve the contractile function of the ventricular muscle and electrical conduction time, as well as repair the heart partly.

关 键 词：微电极阵标测技术 心肌梗死 胶原 组织工程心肌 场电位 激活-恢复时间 

分 类 号：R318[医药卫生—生物医学工程]