候选致病岛Uu146～Uu170基因在不孕不育患者解脲支原体上的鉴定和分析  

Identification and analysis of a putative pathogenicity island Uu146-Uu170 in Ureaplasma urealyticum collected from sterile patients

作　　者：黄珺[1] 张钧[1] 潘建平[2] 宋铁军[1] 谢鑫友[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院检验科,杭州310016 [2]浙江大学医学院病原微生物系

出　　处：《中华医学杂志》2012年第44期3122-3124,共3页National Medical Journal of China

基　　金：国家自然科学基金（81171629）;浙江省自然科学基金（Y2080234）

摘　　要：目的鉴定和分析解脲支原体（Uu）候选致病岛（PAI）Uu146-Uu170基因，并探讨其与不孕不育症的关系。方法收集女性健康组Uu51株、不育女性疾病组Uu57株；男性健康组Uu42株、不育男性疾病组Uu38株。对Uu进行生物分群，PCR扩增PAI。用Fisher精确检验法比较临床表现型、生物群分布和PAI分布之间的关系。结果女性健康组和疾病组的生物1群分别占68．6％（35／51）和73．7％（42／57），PAI的阳性率分别为15．7％（8／51）和12．3％（7／57），健康组与疾病组差异均无统计学意义（均P〉0．05）；男性健康组和疾病组的生物1群分别占71．4％（30／42）和65．8％（25／38），PAI的阳性率分别为21．4％（9／42）和18．4％（7／38），2组间差异均无统计学意义（均P〉0．05）。但女性和男性疾病组的生物2群中PAI的阳性率（5／15和5／13）显著高于生物1群中PAI的阳性率（2／42和2／25）；而女性和男性健康组中的生物1群与生物2群中PAI的阳性率差异均无统计学意义（均P〉0．05）。结论此PAI单独存在不能改变Uu对不孕不育的致病力，但可能为与Uu生物2群相关的致病因子。Objective To identify and analyze a putative pathogenicity island （PAI） Uu146 - Uu170 of Ureaplasma urealyticum （Uu） and explore the relationship between PAI and infertility. Methods Fifty-one Uu isolates were collected from healthy females, 57 isolates from sterile females with fallopian tube disease, 42 isolates from healthy males and 38 isolates from sterile males. Biovar-typing was performed based on the gene of multlple-banded antigen （ MBA）. The distribution of PAI was analyzed by PCR. The association of clinical phenotype with biovar type and＇ PAI distribution was calculated by Fisher＇s exact test. Results In female healthy group and female disease group, biovar type 1 account for 68.6% （35/51） and 73.7% （42/57）, whereas the positive rate of PAI were 15.7% （8/51） and 12. 3% （7/57） in the two groups, both difference were not statistically significant. In male healthy group and male disease group, biovar type 1 account for 71.4% （30/42） and 65.8% （25/38） , whereas the positive rate of PAI were 21.4% （9/42） and 18.4% （7/38） in the two groups, both difference were not statistically significant. The positive rate of PAI in biovar type 2 was significantly higher than that in biovar type 1 in the fallopian tube disease and male infertility groups （ 5/15 vs 2/42,5/13 vs 2/25, both P 〈 0. 05 ） whereas no significant difference was found between two biovar types in normal groups （ both P 〉 0. 05）. Conclusions There is insufficient evidence that Uu with presence of PAI is more pathogenic to cause infertility. The Uu strain of biovar type 2 with PAI Uu146 - Uu170 may be associated with infertility.

关 键 词：解脲支原体 基因组岛 基因Uul46~Uul70 生物群 候选致病岛 

分 类 号：R711.6[医药卫生—妇产科学]