应用基质辅助激光电离飞行时间质谱快速鉴定临床酵母菌  

Rapid clinical yeast identification by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

作　　者：靳颖[1] 杨爽风[2] 王俊妨[1] 刘锦[1] 崔云涛[1] 周鑫[1] 赵化冰[2] 

机构地区：[1]武警后勤学院附属医院检验科 [2]武警后勤学院职业与环境危害生物标志物重点实验室,天津300162

出　　处：《中国真菌学杂志》2012年第5期269-272,共4页Chinese Journal of Mycology

摘　　要：目的研究基质辅助激光解析电离飞行时间质谱(Matrix-Assisted Laser Desorption Ionization-Time of Flight MassSpectrometry,MALDI-TOF-MS)用于快速检测鉴定临床分离的酵母菌的可行性。方法应用Bruker MALDI-TOF-MS和VITEK 2-compact系统分别鉴定150株临床分离的酵母菌,结果不一致的菌株通过基因序列测定来鉴定。结果 MALDI-TOF-MS快速准确鉴定出了150株临床酵母菌,鉴定符合率在属水平上为100%,种水平上为94%。结论基于MALDI-TOF-MS鉴定方法具有很好的可重复性和准确性,并且其检测成本较低,实验准备时间很短,MALDI-TOF-MS可以用于临床分离的酵母菌的快速鉴定。Objective To establish a rapid identification method of clinical yeast. Methods Total 150 isolated clinical yeast strains were analyzed by Bruker Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry （MALDI-TOF-MS） and VITEK 2-compact system respectively, and the different results were determined by gene sequencing. Results All 150 clinical yeast strains were identified accurately and rapidly by MALDI-TOF-MS. A total of 100% of isolates were identified correctly at the genus level, with 94% identified correctly at the species level. Conclusion The MALDI-TOF MS-based method was found to be re- producible and accurate, with low consumable costs and minimal preparative time. These results demonstrated that MALDI-TOF-MS could be a rapid technique for identification of clinical yeast.

关 键 词：基质辅助激光解析电离飞行时间质谱 临床酵母菌 快速鉴定 

分 类 号：R446.53[医药卫生—诊断学]