血小板衍化生长因子BB对体外培养肌腱细胞增殖的影响(英文)  

Effect of platelet-derived growth factor-BB on proliferation of tendon cells cultured in vitro

作　　者：谢爱国[1] 梁红伟 

机构地区：[1]南京医科大学第二附属医院烧伤整形外科,江苏省南京市210011 [2]郑州市人民医院整形外科,河南省郑州市450003

出　　处：《中国组织工程研究》2012年第42期7925-7928,共4页Chinese Journal of Tissue Engineering Research

基　　金：the Science and Technology Development Program of Nanjing Medical University,No. 09NJMUM 025;the Science and Technology Foundation of Nanjing,No.ZKX0415~~

摘　　要：背景:细胞增殖和组织修复是一个复杂的过程,这期间有大量生长因子参与,并且这些因子在发挥本身功效时,还相互影响和制约,从而更好的促进细胞增殖和组织修复。目的:了解不同质量浓度的血小板衍化生长因子BB对体外培养肌腱细胞增殖的影响。方法:体外培养并鉴定大鼠肌腱细胞,根据培养液中血小板衍化生长因子BB质量浓度的不同分为对照组(0μg/L)和实验组(1,5,10,20,50,100,150,200,250μg/L),以MTT法检测细胞增殖能力。另外检测对照组与20μg/L血小板衍化生长因子BB组培养12-72h时肌腱细胞的吸光度值,以观察其增殖量效与时间的关系。结果与结论:分离培养的细胞Ⅰ型胶原染色呈阳性,Ⅲ型胶原染色呈阴性,证明为大鼠肌腱细胞。与对照组相比,除250μg/L血小板衍化生长因子BB组外,其他各实验组细胞的吸光度值均升高(P<0.05或P<0.01);并且随血小板衍化生长因子BB质量浓度的增加,其吸光度值先逐渐增大,达一定浓度(20μg/L)后,又逐渐减小。在20μg/L血小板衍化生长因子BB组,肌腱细胞数量从培养12h开始增加,至48h达高峰,在培养24-72h的吸光度值均显著高于对照组(P<0.01)。结果可见血小板衍化生长因子BB在一定质量浓度和时间范围内具有促进肌腱细胞增殖的作用。BACKGROUND：Cell proliferation and tissue repair is a complicated process,accompanied by participation of multiple growth factors.These factors interact with each other to better promote cell proliferation and tissue repair.OBJECTIVE：To investigate the effect of platelet-derived growth factor-BB（PDGF-BB） of different concentrations on proliferation of tendon cells cultured in vitro.METHODS：Rat tendon cells were cultured and identified in vitro.According to concentration of PDGF-BB nutrient solution in culture medium,the cells were divided into control group（0μg/L） and PDGF-BB groups（1,5,10,20,50,100,150,200,250μg/L）.Proliferation of tendon cells was detected by MTT test.The absorbance value of tendon cells in control group and 20μg/L PDGF-BB group after culture for 12,24,36,48,60 and 72 hours were determined to evaluate relationship with proliferation dose-effect and time.RESULTS AND CONCLUSION：The isolated cells were identified to be tendon cells as they were positive for type Ⅰ collagen staining and negative for type Ⅲcollagen staining.Compared with control group,the absorbance values of all groups were all increased,except for 250μg/L PDGF-BB group（P0.05 or P0.01）.Besides,the absorbance value rose gradually with the increase of the concentration of PDGF-BB,and then diminished gradually with the increase of the concentration of PDGF-BB from 20μg/L.The number of tendon cells in 20μg/L PDGF-BB group began to increase when cultured for 12 hours,and reached the highest level at 48 hours,and the absorbance at 24-72 hours was significantly greater than control group（P0.01）.Results suggest that PDGF-BB can promote the proliferation of tendon cells in a definite range of concentration and time.

关 键 词：血小板衍化生长因子BB 肌腱细胞 增殖 细胞培养 体外 

分 类 号：R318[医药卫生—生物医学工程]