缺氧诱导因子1在创伤性深静脉兔模型血栓形成及消退过程中的表达(英文)  

Hypoxia-inducible factor-1 expression in thrombosis and thrombolysis of a rabbit model of traumatic deep vein thrombosis

作　　者：刘海平[1] 赵学凌[1] 吴雪梅[1] 周如丹[1] 李宏昆[1] 李兴国[1] 郑宏宇[1] 

机构地区：[1]昆明医学院第一附属医院骨科,云南省骨科研究中心,云南省昆明市650032

出　　处：《中国组织工程研究》2012年第42期7929-7932,共4页Chinese Journal of Tissue Engineering Research

基　　金：the National Natural Science Foundation of China,No.81060151;the Science and Technology Development Program of Yunnan Province,No.2010BC010~~

摘　　要：背景:深静脉血栓形成及消退过程中的分子机制极其复杂,目前已有研究表明,缺氧与损伤因素参与了深静脉血栓形成及消退过程。目的:观察静脉壁中缺氧诱导因子1在创伤性深静脉血栓模型兔血栓形成及消退过程中的表达变化。方法:将日本大耳白兔采用钳夹双侧股静脉+石膏固定双下肢建立兔创伤性深静脉血栓模型。PCR及ELISA检测股静脉组织中缺氧诱导因子1mRNA、蛋白在兔创伤性深静脉血栓建模后表达的变化。结果与结论:模型兔创伤后24h,经B超监测,血栓形成率为58%;血栓栓子随造模后时间延长逐渐缩小机化,创伤后第5天开始经B超监测模型组血栓形成的血管腔有断续血流信号。模型兔股静脉组织中缺氧诱导因子1在造模后1,3,5d表达逐渐升高(P<0.05或P<0.01),此后7-14d表达逐渐下降(P<0.05或P<0.01)。结果证实,在创伤性深静脉血栓形成过程,缺氧诱导因子1表达上调,在血栓形成后的溶解及机化再通过程中,缺氧诱导因子1表达下调。BACKGROUND：The molecular mechanism of deep vein thrombosis and further regression is extremely complex.Researches show that hypoxia and injury factors are involved in deep vein thrombosis and regression.OBJECTIVE：To investigate changes in hypoxia inducible factor（HIF）-1 expression during deep vein thrombosis and regression in a rabbit model of traumatic deep vein thrombosis（TDVT）.METHODS：The bilateral femoral vein of rabbits was clamped and both lower extremities were fixed with gypsum to establish model of TDVT.SYBR Green I fluorescence real-time quantitative PCR and ELISA dynamic detection were used to determine the change of HIF-1 mRNA and protein expression in femoral vein in the rabbits with TDVT.RESULTS AND CONCLUSION：At 24 hours post-injury,B-ultrasonography showed that thrombosis rate was 58% and thromboembolus gradually reduced and be organized with time;from the 5 th day intermittent flow signals were monitored in lumen of blood vessel with thrombosis of model group.HIF-1 expression in vein of model group gradually increased（P0.05 or P0.01）,and reduced between 7-14 days（P0.05 or P0.01）.Those findings indicate that HIF-1 expression is upregulated during TDVT,but downregulated during thrombolysis and organization.

关 键 词：创伤 深静脉血栓 缺氧诱导因子1 组织构建 

分 类 号：R318[医药卫生—生物医学工程]