骨髓瘤细胞裂解物致敏的DC-CIK细胞抗骨髓瘤细胞作用  

Anti-myeloma cell effect by dentritic cells loading antigen of myeloma cells combine cytokine induced killer cells

作　　者：罗云[1] 白凤霞[1] 张萍[1] 娄世锋[1] 

机构地区：[1]重庆医科大学附属第二医院血液科,重庆400010

出　　处：《中国免疫学杂志》2012年第11期986-988,991,共4页Chinese Journal of Immunology

基　　金：重庆市卫生局科研基金项目(No.2010-2-161)

摘　　要：目的:研究负载骨髓瘤细胞裂解物的健康供者树突状细胞(Dendritic cells,DCs)联合细胞因子诱导杀伤细胞(Cytokine induced kil1er cells,CIK)体外对骨髓瘤U266细胞杀伤作用的影响。方法:通过血细胞分离机采集健康供者单个核细胞100 ml用于体外培养,选取贴壁细胞进行DC细胞培养,悬浮细胞用于CIK细胞培养,DC细胞培养7日后用骨髓瘤U266细胞裂解物致敏,然后与CIK细胞共培养7日,用倒置显微镜、扫描电镜观察细胞形态,流式细胞仪检测细胞表型,乳酸脱氢酶释放法检测共培养细胞对U266细胞杀伤作用。结果:CIK细胞大部分形态为圆形,少数梭形,DC细胞有典型细胞突起,流式细胞仪分析培养的CIK细胞高表达CD3,培养后CD3和CD56共表达细胞明显升高,在致敏后DC细胞表达CD86、CD80,共培养细胞对U266细胞杀伤作用强于单独CIK细胞作用。结论:瘤细胞裂解物致敏的健康供者DC-CIK细胞能显著提高对瘤细胞的杀伤性,在患者干细胞移植后是否可应用以清除残留病变,值得进一步研究应用。Objective：To investigate the effect of donors cytokine induced killer cells cells combine DCs loaded with whole antigens of U266 cells on the U266 cells survival in vitro.Methods：100 ml peripheral blood mononuclear cells were isolated from healthy donor by employing blood cell separator.Adherent cells were cultured for DCs and suspension cells for CIK cells culture.After 7 days,DCs were loaded with whole antigens of U266 cells and added CIK cells to co-culture.To observe the cells morphous by inverted microscope and scanning electron microscope.The immunophenotype assay was used by the FCM.To evaluate killing rate of U266 cells by lactic dehydrogenase release.Results：Majority cells were round and minority cells were fusiform in the CIK cells,the DC cells had typical prominence.The majority CIK cells express CD3 positive,after cultured CD3 and CD56 coexpression positive cells added.DCs loaded with whole antigen of U266 cells,CD80 and CD86 expression positive cells added.The results showed that DCs loaded with whole antigen of U266 cells co-culture with CIK cells can kill more amount U266 cells than CIK solo.Conclusion：The CIK cells combine DCs loaded with whole antigen of myeloma cells can obtain more powerful anti-myeloma effect.It may be eliminate minimal residual desease after stem transplantation and be worth research in future.

关 键 词：骨髓瘤 树突状细胞 免疫疗法 过继 细胞因子诱导杀伤细胞 

分 类 号：R392.12[医药卫生—免疫学]