香菇多糖和黄芪多糖对免疫抑制小鼠免疫功能调节的研究  

Effect of Lentinan and Astragalus Polysaccharides on immunological function in immunosuppressed mice

作　　者：颜爱[1] 李波[1] 李润成[1] 喻凯[1] 余兴龙[1] 

机构地区：[1]湖南农业大学动物医学院,长沙410128

出　　处：《中国免疫学杂志》2012年第11期999-1001,1005,共4页Chinese Journal of Immunology

基　　金：长沙科技计划重大专项(K0902007-21)

摘　　要：目的:研究香菇和黄芪多糖对免疫抑制小鼠免疫功能的调节作用。方法:通过检测环磷酰胺免疫抑制模型小鼠的迟发型变态反应程度(DTH,足跖增厚法)、脏器指数、NK细胞活性(乳酸脱氢酶释放法)、淋巴细胞增殖能力(MTT试验)以及IL-2、IL-4、IL-10、IFN-γ水平变化(ELISA试剂盒)来评价香菇和黄芪多糖对免疫抑制小鼠免疫功能的影响。结果:实验表明,环磷酰胺能显著抑制正常小鼠DTH程度、NK细胞活性及细胞因子分泌水平(均为P<0.05);香菇和黄芪两种多糖能显著恢复DTH程度(P<0.05),极显著增高NK细胞包活性(P<0.01),同时能提高血清中IL-2、IL-4、IL-10、IFN-γ的含量。结论:香菇及黄芪多糖对免疫抑制小鼠具有显著的免疫功能恢复效果;香菇多糖的免疫调节效应明显优于黄芪多糖。Objective：To investigate the effect of Lentinan and Astragalus polysaccharides on immunoregulation in mice.Methods：After oral administration of the two polysaccharides,immune effect of the two polysaccharides on the whole immunity was investigated through delayed type hypersensitivity（DTH）,visceral organs index,NK cell activity（lactate dehydrogenase releasing method）,lymphocyte proliferation capacity（MTT） and the level of IL-2,IL-4,IL-10,IFN-γ（ELISAs） of immunosuppressed mice induced by cyclophosphamide.Results：Cyclophosphamide-treated mice showed a significant decrease in DTH,NK cell activity and the serum level of cytokines（each was P0.05）;Both Lentinan and Astragalus polysaccharides accelerated recovery of DTH（P0.05）,as well as NK cell activity（P0.01） and restored the level of IL-2,IL-4,IL-10,IFN-γ in the serum.Conclusion：Both Lentinan and Astragalus polysaccharides can significantly stimulate the immune function in mice with immunologic suppression;the immunological enhancement effect of Lentinan is obviously better than Astragalus polysaccharides.

关 键 词：免疫调节 黄芪多糖(APS) 香菇多糖(LNT) Th1 Th2免疫平衡 

分 类 号：R285.5[医药卫生—中药学]