强直性脊柱炎患者外周血TCR Vα CDR3谱系基因扫描分析研究  

Study on the TCR Vα CDR3 Lineage in peripheral blood by GeneScan in patients with ankylosing spondylitis

作　　者：魏克娜[1] 余伍忠[1] 徐新玉[1] 张璐[1] 邹红云[1] 

机构地区：[1]兰州军区乌鲁木齐总医院临床医学研究所,乌鲁木齐830000

出　　处：《中国免疫学杂志》2012年第11期1009-1013,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(81172840);兰州军区医药卫生科研基金资助项目(CWS10JA21)经费资助

摘　　要：目的:探讨强直性脊柱炎(AS)患者外周血T细胞受体α链可变区互补决定区3(TCR Vα CDR3)谱系多态性,为AS的免疫发病机制的研究提供实验基础。方法:采用反转录-聚合酶链反应(RT-PCR)扩增34个TCR Vα亚家族,经免疫扫描谱型技术分析AS患者外周血单个核细胞(PBMC)中T细胞TCR Vα CDR3的谱系漂移情况。结果:5例正常健康对照PB-MC TCR Vα CDR3谱型多数呈高斯分布。所有AS患者外周血T细胞均出现多个TCR Vα亚家族谱型的异常改变,异常峰型包括:单峰、寡峰/寡峰趋势、偏峰和不规则异常峰型。34个TCR Vα亚家族中,共有17个亚家族在少数患者中的扫描谱型呈单峰即单克隆增生。结论:AS患者PBMC TCR Vα CDR3谱系具有显著多态性,表明T细胞在AS免疫发病机理中扮演重要角色。单/寡克隆增生的T细胞有可能是AS发病中的自身反应性T细胞,将为AS的发病机制的进一步研究提供基础依据。Objective：To study T cells lineage polymorphism of TCR Vα CDR3 in peripheral blood of ankylosing spondylitis（AS） patients so as to provide experimental basis to immune pathogenesis research in AS.Methods：T cells TCR Vα 34 subfamilies in AS patients PBMC were amplified by RT-PCR method,then TCR Vα CDR3 lineages polymorphism were analyzed by immunization scanning spectrum.Results：TCR Vα CDR3 scanning spectrum of 10 AS patients all showed abnormal distribution peak,including monoclonal,oligoclonal/oligoclonal trend,skewing peak and irregular abnormal peak type subfamilies.A total of 17 subfamilies appeared that monoclonal proliferation of a single peak in 10 cases patients,the frequency in descending order were： Vα19 subfamily that monoclonal proliferation of a single peak in three patients;each Vα32,Vα7 and Vα1.2 showed in 2 patients with a single peak;following showed a single peak.Conclusion：TCR Vα CDR3 lineage have significant polymorphism and spectrum drift characteristics in AS patients peripheral blood,which further indicate that T cells play an important role in immunological pathogenesis in AS.Monoclonal/oligoclonal expansion of T cells may be autoreactive T cells in the pathogenesis of AS,and this study provides a foundation for further research into the mechanism of AS.

关 键 词：强直性脊柱炎 T细胞受体 Vα亚家族 互补决定区3 免疫扫描谱型分析 

分 类 号：R392[医药卫生—免疫学]