人β-防御素2联合乙肝疫苗对小鼠免疫效应的研究  

The immunity study of the strengther effective of human beta-defensin-2 on HBsAg vaccine in BALB/c mice

作　　者：曾文兴[1] 苏虹[1] 王美珍[2] 刘玉琳[1] 王福财[1] 胡有长[1] 江丽霞[3] 施桥发[1] 

机构地区：[1]南昌大学医学院,南昌330006 [2]南昌大学医院,南昌330006 [3]赣南医学院第一附属医院检验科,赣州341000

出　　处：《中国免疫学杂志》2012年第11期1023-1027,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(No.30760280);江西省科技厅社会发展重点项目(No.2007BS12802);江西省卫生厅科技计划项目(No.20072016)资助

摘　　要：目的:探讨人β-防御素2加强乙肝疫苗对小鼠免疫应答的作用。方法:采用乙肝疫苗2μg/次/只联合HBD2基因真核表达质粒pEGF-C1/HBD2 100μg/次/只,按间隔2周、共免疫3次的左后肢股四头肌肌肉接种方案免疫BALB/c小鼠,同时设立相应的对照。免疫后用双抗原夹心ELISA法测小鼠血清HBsAb水平。采用流式细胞仪检测脾脏CD4+T和CD8+T细胞百分率及CD4+/CD8+比值。应用CCK8试剂盒检测脾淋巴细胞在不同抗原刺激下的增殖反应。结果:pEGF-C1/HBD2质粒联合乙肝疫苗免疫小鼠抗体水平明显升高,CD4+T细胞百分率比其他免疫组都高,脾淋巴细胞体外增殖实验明显。与其他各免疫组相比,pEGF-C1空质粒联合乙肝疫苗免疫组抗体水平和淋巴细胞体外增殖情况及CD4+T细胞百分率都较低。结论:人β-防御素-2对乙肝疫苗在小鼠中的免疫功能有一定增强作用。Objective：To explore whether the beta-defensin-2 can strengthen the immune effectives of hepatitis B vaccine. Methods： Mice were immunized with both 2 μg hepatitis B vaccine and 100 μg pEGF-C1/HBD2 containing I-IBD2 eukaryotic expression plasmid by intramuscular injection in the left tibialis anterior muscle, and this kind of immunization was carried on 3 times by 2 weeks interval. The corresponding control group was set up at the same time. Serum antibody level in mice were measured by sandwich ELISA, and CD4 ＋ and CD8 ＋ T cell percentage of spleen and ratio of CD4 ＋/CD8 ＋ were measured by flow cytometry, and the proliferation of spleen lymphocytes was tested by CCK8 assay under the stimulation from ConA or hepatitis B vaccine in vitro. Results：The lev- el of serum HBsAb from the hepatitis B vaccine combined with pEGF-C1/HBD2 immunized mice was higher than other groups. The proliferation in vitro and the CD4 ＋ T cell percentage of spleen lymphocytes from the mice immunized with the plasmid pEGF-C1/HBD2 and hepatitis B vaccine were also higher than other groups. And all these figures from the group which was immunized with the empty plasmid pEGF-C1 plus hepatitis B vaccine were lower than others. Concluslon：Beta-defensin-2 may play a strengthen role of immunization of hepatitis B vaccine.

关 键 词：乙肝疫苗 人β-防御素2 细胞免疫 佐剂 

分 类 号：R392.1[医药卫生—免疫学]