乙肝核心抗原DNA疫苗的构建及其免疫原性分析  

Construction of hepatitis B virus core antigen DNA vaccine and its immunogenic analysis

作　　者：陈蕊雯[1] 林懿[1] 戴建新[1] 周凤娟[1] 孙树汉[1] 

机构地区：[1]第二军医大学基础医学部医学遗传学教研室,上海200433

出　　处：《第二军医大学学报》2000年第6期501-503,共3页Academic Journal of Second Military Medical University

基　　金：国家"863"计划资助!项目 (10 1-0 6 -0 5 0 4)

摘　　要：目的 :构建 HBV核心抗原 DNA疫苗 ,并探讨其在实验动物体内的表达及其免疫原性。方法 :采用 PCR法从 HBV全基因 DNA中获得核心抗原 DNA片段 ,并将其重组到 pc DNA3载体。以此为候选疫苗分别免疫小鼠和树 ,并检测其抗HBc Ig G。结果 :构建了乙肝核心抗原候选核酸疫苗 pc DNA3- HBc。免疫接种该疫苗后第 2周抗 HBc Ig G水平开始升高 ,小鼠至第 9周 ,树至第 7周升至峰值。结论 :构建的 pc DNA3- HBcObjective: To construct DNA vaccine for HBV core antigen and study its expression and immunogenicity in animals. Methods:HBc DNA fragment was obtained from HBV whole genomic DNA with PCR and was cloned into DNA vaccine vector pcDNA3. The constructed candidate for DNA vaccine was immunized in mice and tree shrew and it's anti HBc IgG was detected. Results: A recombinant DNA vaccine pcDNA3 HBc was constructed. Anti HBc IgG was detected 2 weeks after immunization and continued to increase until week 9 in mice and week 7 in tree shrew. Conclusion: DNA vaccine pcDNA3 HBc is expressed in vivo and induces humoral response.

关 键 词：DNA 乙型肝炎核心抗原 乙型肝炎 HBV 

分 类 号：R512.62[医药卫生—内科学]