Spectroscopic study on the interaction between[Hg(SCN)_4]^(2-) and hemoglobin  

作　　者：Lun Fu Tian Zhong Fang Liu Ling Kong Xiao Li Hu Shao Pu Liu 

机构地区：[1]Education Ministry Key Laboratory on Luminescence and Real-Time Analysis,School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China [2]Institute of Mechanical Manufacturing Technology,Chinese Academy of Engineering Physics,Mianyang 621900,China

出　　处：《Chinese Chemical Letters》2012年第11期1283-1286,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.20875078)

摘　　要：The interaction between [Hg（SCN）4]^2- and hemoglobin （Hb） under conditions that simulate a physiological environment was investigated by UV-vis spectroscopy, fluorescence spectroscopy, resonance Rayleigh scattering （RRS） spectroscopy and circular dichroism （CD） spectroscopy. The results obtained from the change of UV-vis and CD spectra, the quenching of Hb fluorescence and the enhancement of RRS intensity proved that a 10：1 type complex was formed between [Hg（SCN）4]^2- and Hb. The possible mechanism suggested for the interaction was that ten Hg（SCN）4]^2- anions entered the four subunits of a Hb molecule to react with some residues to form an adduct by coordination and electrostatic forces. The coordination of [Hg（SCN）4]^2- with Trp was the major cause of the fluorescence quenching of Hb.The interaction between [Hg（SCN）4]^2- and hemoglobin （Hb） under conditions that simulate a physiological environment was investigated by UV-vis spectroscopy, fluorescence spectroscopy, resonance Rayleigh scattering （RRS） spectroscopy and circular dichroism （CD） spectroscopy. The results obtained from the change of UV-vis and CD spectra, the quenching of Hb fluorescence and the enhancement of RRS intensity proved that a 10：1 type complex was formed between [Hg（SCN）4]^2- and Hb. The possible mechanism suggested for the interaction was that ten Hg（SCN）4]^2- anions entered the four subunits of a Hb molecule to react with some residues to form an adduct by coordination and electrostatic forces. The coordination of [Hg（SCN）4]^2- with Trp was the major cause of the fluorescence quenching of Hb.

关 键 词：[Hg（SCN）4]^2- HEMOGLOBIN Toxicological evaluation Molecular spectroscopy 

分 类 号：O433[机械工程—光学工程] Q51[理学—光学]