乙型肝炎病毒外膜大蛋白检测的临床意义  

The clinical significance of the detection of hepatitis B virus large surface protein for the diagnosis and treatment

作　　者：纪新梅[1] 李海珠[1] 杜辉[1] 仇思武[1] 赵柏良[1] 

机构地区：[1]肇庆市第一人民医院检验科,广东省肇庆526020

出　　处：《中国基层医药》2012年第24期3681-3682,共2页Chinese Journal of Primary Medicine and Pharmacy

基　　金：广东省肇庆市科技创新计划项目（2012E184）

摘　　要：目的探讨乙型肝炎病毒外膜大蛋白（HBV—LP）与乙型肝炎病毒脱氧核糖核酸（HBV—DNA）的相关性，动态检测俩项指标，分析其在诊断乙型肝炎和临床治疗，妒的意义。方法采用酶联免疫吸附试验和实时荧光定量聚合酶链反应技术对230例乙型肝炎患者血清标本进行HBV—LP和HBV-DNA平行检测。结果乙型肝炎病毒感染患者血清HBV—LP含量与HBV—DNA拷贝数间呈正相关（r=0．84，P〈0．01），230例乙肝病毒感染患者血清标本中，HBV-LP阳性率为84.78％，HBV-DNA阳性率为84．35％，两者差异无统计学意义（P〉0．05）。55例HBeAg阴性血清标本中，HBV—LP与HBV-DNA的阳性检出率分别为63．63％（35／55）、58．18％（32／55），两者差异无统计学意义（P〉0．05）。HBV—DNA阳性194例中，HBV—LP阳性率（82．47％）明显高于HBeAg阳性率（52．06％）（x2=80：3，P〈0．01）。结论HBV—LP及HBV-DNA是判断乙型肝炎病毒感染患者体内病毒复制程度的重要指标，尤其是HBeAg阴性患者体内病毒复制及预后判断有价值的血清学检测指标，为临床诊断及治疗提供可靠的实验室数据。Objective To explore the con＇elation between hepatitis B virus large surface pro, rein （HBV-LP） and hepatitis B virus deoxyribonucleic acid （HBV-DNA）, analyze the clinical significance of detecting the two index dynamically for diagnosis and trealment of hepatitis B. Methods Enzyme linked immunosorbent assay （ELISA） and fluorescent quantitation polymerase chain reaction （FQ-PCR） were used to detect the levels of HBV-LP and HBV- DNA in serum specimen of 230 hepatitis B patients. Results There was a positive correlation between the content of HBV-I,P and copy numbers of HBV-DNA in serum of hepatitis B positive cases （ r = 0. 84, P 〈 0. 01 ）. The positive rate was 84.78% in HBV-LP and 84.35% in ttBV-DNA of230 HBV positive cases. As a result,there was no significant difference （P 〉0.05 ） in this study. Therefore, the positive rate was 63.63% （35/55） in HBV-LP and 58.18% （32/55） in HBV-DNA of 55 HBV negative cases. There was also no significant difference （P 〉 0. 05） in this study. However,the positive rate of HBV-LP （82.47%） was higher than HBE （52.06%） in the 194 HBV-DNA positive cases. There was significamly different （P 〈0.01）. Conclusion HBV-LP and HBV-DNA are the significant index of the degree of hepatitis B virus replication in hepatitis B positive cases, especially in determining the virus replication and prognosis of treatment in HBe Ag negative,providing the reliable laboratory data for the clinical diagnosis and treatment.

关 键 词：乙肝病毒外膜大蛋白 肝炎病毒 乙型 病毒复制 

分 类 号：R51[医药卫生—内科学]