枸杞多糖对脑缺血再灌注损伤小鼠脑组织的保护作用  

Neuroprotective Effects of LBP on Brain Ischemic Reperfusion Injury

作　　者：郝银菊[1] 王红波[1] 马琳 王腾飞[1] 王洁 李玉香[4] 余建强[1,3] 

机构地区：[1]宁夏医科大学药学院,银川750004 [2]宁夏颅脑疾病重点实验室,银川750004 [3]宁夏回族自治区医学科学研究所,银川750004 [4]宁夏医科大学护理学院,银川750004

出　　处：《宁夏医科大学学报》2012年第10期977-980,F0002,共5页Journal of Ningxia Medical University

基　　金：国家自然科学基金(309605060;81160524);宁夏自然科学基金(NZ11212);宁夏医科大学重点项目(XM2011017)

摘　　要：目的探讨枸杞多糖对脑缺血再灌注损伤小鼠脑组织的保护作用。方法枸杞多糖10、20、40mg.kg-1灌胃给药,1天1次,连续7d,于末次给药1h后采用线栓法制作小鼠局灶性脑缺血再灌注模型,通过Bed-erson、Turgut评分法对各组小鼠进行神经功能缺失体征评分;单极引导法观察枸杞多糖对各组小鼠脑缺血前、缺血15min、再灌注15、45、90min后的脑电图变化;TTC染色法测定脑梗死体积。结果与模型组比较,LBP(20、40mg.kg-1)组和尼莫地平组再灌注90min,小鼠EEG幅度分别恢复到正常水平的54.4%、85.9%、75.4%(P<0.05或<0.01);LBP(20、40mg.kg-1)组和尼莫地平组与模型组比较,小鼠神经功能评分明显降低(P<0.01),即可明显改善脑缺血再灌注小鼠的行为障碍;与模型组比较,LBP(20、40mg.kg-1)组小鼠脑梗死面积明显缩小(P<0.05或0.01)。结论枸杞多糖对脑缺血再灌注损伤小鼠脑组织有较好的保护作用。Objective To investigate whether LBP had a protective effect on cerebral ischemic reperfusion in- jury in mice. Methods LBP （ 10, 20, 40 mg·kg-1） was administered intragastrically daily for seven consecutive days. The cerebral artery occlusion/ reperfusion （MCAO/R） model was established at l h after the last dose via suture ligation. Bederson, Turgut score were employed to assesse the neurological deficit signs and unipolar lead to monitor the change of electroencephalograph （EEG） before and 15 rain after ischemia as well as 15,45,90 rain after ischemia - repel＇fusion was monitored by unibipolar lead of model SMUP - E Bio - electric Signals Processing System on mice. The infarction area of brain was determined in brain slices with 2% solution of 2,3,5 -triphenyl tetrazolium chloride （TTC）. Results Compared with model group, mice EEG amplitude of LBP （20,40mg·kg-1） group and nimodipine group were restored to 54.4% , 85.9 % , 75.4% of the normal level after reperfusion 90 rain, respectively （P 〈 0.05 or P 〈 0.01 ）. Compared with model group, neurological score of cerebral ischemia- reperfusion in LBP （20,40 mg/kg） group was significantly reduced （P 〈 0.01 ）. Conclusion LBP possesses an optimal protective effect on the cerebral ischemia - reperfusion injury.

关 键 词：枸杞多糖 脑缺血再灌注损伤 神经保护作用 小鼠 

分 类 号：R285.5[医药卫生—中药学]