Eg.ferritin抗血清介导的体外杀伤细粒棘绦虫原头节作用的观察  

An Observation in Vitro of Recombinant Ferritin Protein Antiserum Impaired the Protoscolex of Echinococcus Granulosus

作　　者：王娅娜[1,2,3] 张炎[1] 朱佳[1] 

机构地区：[1]宁夏医科大学医学科学技术研究中心,银川750004 [2]宁夏医科大学医学遗传学与细胞生物学教研室,银川750004 [3]宁夏回族自治区遗传与生殖重点实验室,银川750004

出　　处：《宁夏医科大学学报》2012年第10期1000-1004,共5页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金(NZ0993)

摘　　要：目的体外观察细粒棘球绦虫重组铁蛋白(Eg.ferritin)抗血清对细粒棘球蚴原头节(PSC)的杀伤作用。方法用纯化的Eg.ferritin免疫新西兰家兔制备抗血清,以不同浓度的抗血清与细粒棘球绦虫原头蚴体外共培养,光镜观察培养不同时间点细粒棘球原头节的变化,透射电子显微镜观察原头节超微结构的改变。结果抗血清组的原头蚴的死亡率及破坏程度与抗血清的浓度呈正相关,与对照组相比,抗血清对原头节有杀伤作用(P<0.01)。在20%抗血清组中,原头节内部被大量空泡及髓样结构代替或无结构。结论细粒棘球蚴重组铁蛋白抗体对细粒棘球绦虫有明显的杀伤作用。Objective To observe the impaired the effect of recombinant ferritin protein antiserum on the pro-toscolex （PSC） of Echinococcus granulosus in vitro. Methods Antiserum was made from New Zealand rabbits with purified Eg. ferritin immunization. Antiserum was divided into three different group with 10%, 15%, 20% concentration. PSC were cultured in the three antiserum groups. Living situation and mortality of PSC was investigated through microscope in different time points and sub structure of PSC was observed through transmission electronic microscope （TEM）. Results Impairment of PSC was positively related to concentration of anti - serum（ P 〈 0.01 ） , Lots of vacuole and myelinfigures were found inside of PSC in 20% anti - serum group. Conclusion The results confirmed that Eg. ferritin could impaire PSC in vitro.

关 键 词：重组铁蛋白 抗血清 细粒棘球原头节 

分 类 号：R392[医药卫生—免疫学]