调补肺肾三法治疗慢性阻塞性肺疾病大鼠疗效及远后效应  

Efficacy and long-term effect of three therapies of invigorating lung and kidney for rat with stable COPD

作　　者：李素云[1] 李亚[1] 李建生[1,2] 王元元[1] 邓丽[2] 田燕歌[2] 姜素丽[2] 王英[2] 

机构地区：[1]河南中医学院第一附属医院呼吸病研究所,郑州450000 [2]河南中医学院老年医学研究所,郑州450008

出　　处：《中华中医药杂志》2012年第12期3116-3121,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然基金重点项目(No.81130062);国家自然基金面上项目(No.30973743;No.81173236);2010年度河南省科技创新人才项目(No.104200510004)~~

摘　　要：目的:评价调补肺肾(补肺健脾、补肺益肾、益气滋肾)三法治疗慢性阻塞性肺疾病(COPD)稳定期大鼠的疗效和远后效应。方法:大鼠随机分为对照组、模型组、补肺健脾组、补肺益肾组、益气滋肾组和氨茶碱组,采用香烟熏吸联合细菌感染法制作COPD稳定期模型,于第9周起分别给予补肺健脾方、补肺益肾方、益气滋肾方及氨茶碱至第20周。于第20、32周分批取材,观察大鼠肺功能和肺组织形态学变化。结果:模型组潮气量(TV)、呼气峰流速(PEF)和50%潮气量呼气流量(EF50)较对照组显著降低(P<0.01),病理损伤严重。第20、32周时,3个中药组上述指标均较模型组明显改善(P<0.05,P<0.01)。第20周时,3个中药可明显减轻肺组织病理损伤,表现在改善大鼠支气管壁增厚、支气管狭窄、肺泡破裂和融合、肺小动脉壁增厚(P<0.05,P<0.01),并在32周仍有显著疗效(P<0.05,P<0.01)。结论:调补肺肾三法治疗COPD稳定期大鼠具有良好的疗效和远后效应。Objective： To evaluate the efficacy and long-term effect in rat with stable chronic obstructive pulmonary disease（COPD） treated with the three Tiao-Bu Fei-Shen（nourishing lung and kidney） therapies,including Bu-Fei Jian-Pi（invigorating lung and spleen）,Bu-Fei Yi-Shen（supplementing lung and kidney） and Yi-Qi Zi-Shen（nourishing qi and kidney）.Methods： Rats were randomLy divided into control group,model group,Bu-Fei Jian-Pi group,Bu-Fei Yi-Shen group,Yi-Qi Zi-Shen group and aminophyline group.Repeated smoke inhalations and bacterial infections were used to establish the stable COPD rat model.From the ninth to twentieth week,the COPD rats were treated with normal saline,Bufei Jianpi granules,Bufei Yishen granules,Yiqi Zishen granules or aminophyline respectively.Half of animals were sacrificed at the 20th week,and the survival fed under normal condition until 32th week,pulmonary function and lung tissue histological changes were observed.Results： Compared with control rats,the tidal volume（VT）,peek expiratory flow（PEF） and 50% tidal volume expiratory flow（EF50） decreased significantly in the COPD rats（P0.01）,and showed obvious histological changes.At the 20th and 32nd week,TV,PEF and EF50 in the three Chinese medicines treated groups improved compared with model group,and histological damage in lungs reduced significantly,such as bronchial wall thickening,bronchial stenosis,alveolar rupture and fusion,pulmonary artery wall thickening（P0.05 or P0.01）.Conclusions： The three Tiao-Bu Fei-Shen therapies can significantly improve pulmonary function and lung pathological impairment in stable COPD rat,and has a better long-term effect than aminophylline.

关 键 词：补肺健脾 补肺益肾 益气滋肾 慢性阻塞性肺疾病 疗效 远后效应 

分 类 号：R259[医药卫生—中西医结合]